Anti-cholinesterases and memory improving effects of Vietnamese Xylia xylocarpa
© The Author(s) 2016
Received: 27 April 2016
Accepted: 28 July 2016
Published: 3 August 2016
Alzheimer’s disease (AD) is the most common cause of dementia among the elderly and is characterized by loss of memory and other cognitive functions. An increase in AChE (a key enzyme in the cholinergic nervous system) levels around β-amyloid plaques and neurofibrillary tangles is a common feature of AD neuropathology. Amnesic effects of scopolamine (acetylcholine receptor antagonist) can be investigated in various behavioral tests such as Morris water maze, object recognition, Y-maze, and passive avoidance. In the scope of this paper, we report the anti-AChE, anti-BChE properties of the isolated compound and the in vivo effects of the methanolic extract of Xylia xylocarpa (MEXX) on scopolamine-induced memory deficit.
In further phytochemistry study, a new hopan-type triterpenoid, (3β)-hopan-3-ol-28,22-olide (1), together with twenty known compounds were isolated (2–21). Compound 1, 2, 4, 5, 7–9, and 11–13 exhibited potent acetylcholinesterase (AChE) inhibitory activity in a concentration-dependent manner with IC50 values ranging from 54.4 to 94.6 μM. Compound 13 was also shown anti-butyrylcholinesterase (BChE) activity with an IC50 value of 42.7 μM. The Morris water Y-maze, Y-maze, and object recognition test were also carried out.
It is noteworthy that MEXX is effective when administered orally to mice, experimental results are consistent with the traditional use of this medicinal plant species.
Alzheimer’s disease (AD), a degenerative brain disorder leading to dementia, is one of the most common disorders of old age, affecting nearly 4 million individuals in the US. Typical clinical features of Alzheimer’s disease are memory loss, language deterioration, reduced visual space, sensation disorders and epilepsy advocacy gradual progression of terminal illness [1, 2]. There are several theories about the cause of Alzheimer’s disease, in which the theory about the decline of acetylcholine is the most widely accepted and is the basis for the current development of the drugs of Alzheimer’s disease. The research on Alzheimer’s patients demonstrated that cholinergic abnormalities correlated with the degree of memory and cognitive impairment [2, 3]. These findings have led to the treatment of Alzheimer’s disease by increasing the activity of the cholinergic system (acetylcholinesterase, AChE, inhibitory mechanism) [2, 3]. Recently, some research found that AChE is also related to the formation of amyloid plaques and neurofibrillary tangles .
Xylia xylocarpa (Roxb.) Taub. is a perennial tree belonging to the family Fabaceae, which is sparsely distributed in Burma, Vietnam, Cambodia, and India. In Vietnam, X. xylocarpa is known as “Cam Xe”; the bark, heartwood, and flower have been used as Vietnamese traditional medicines for the treatment of dementia, duodenal, stomach pain, vomiting, diarrhoea, gonorrhoea, leprosy, and rheumatism . Previously, the chemical constituents of the wood of X. xylocarpa have been reported some flavan-3-ols including monomer, dimer, and trimer of epiafzelechin . Our preliminary screening study also revealed that the methanolic extract of the wood of X. xylocarpa exhibited significant AChE and BChE (butyrylcholinesterase) inhibitory activities with IC50 values of 16.17 and 7.13 μg/mL, respectively. In the present study, we report the cognitive-enhancing effect of the methanolic extract of X. xylocarpa (MEXX) on amnesic mice induced by scopolamine in vivo. In addition, the isolation of MEXX was carried out, a new hopan-type triterpenoid, (3β)-hopan-3-ol-28,22-olide (1) was isolated together with twenty known compounds (2–21). We also reported the anti-AchE, anti-BChE properties of the isolated compound herein.
Results and discussions
Compound 1 exhibited an [M + H]+ and [M + Na]+ peak at m/z 457.3674 and 479.3482, respectively, in the positive HR-ESI-MS, corresponding to the molecular formula C30H48O3. The 13C NMR spectrum of compound 1 showed thirty carbon signals, including one lactone carbonyl carbon (δ C 175.9), one hydroxylated methine (δ C 79.1), and one oxygenated tertiary carbon (δ C 83.4). Together with the HSQC analysis, all the remaining carbon signals were identified as five methines, ten methylenes, five quaternary carbons and seven tertiary methyl groups. The 1H NMR spectrum of compound 1 also exhibited an oxygenated methine proton signal at δ H 3.19 (dd, J = 11.4 and 4.8 Hz, H-3) and seven singlet methyl signals (δ H 1.46, 1.33, 0.96, 0.94, 0.93, 0.83, 0.76). Based on the analysis of these spectra, compound 1 was suggested to be an hopan-type triterpenoid [25, 26].
1H and 13C NMR data for (3β)-hopan-3-ol-28,22-olide (1) in CDCl3
δ C, type
δ H (J in Hz)
3.19, dd (11.4, 4.8)
2.41, dt (13.3, 3.5)
2.13, t (4.4)
Cholinesterase inhibitory activity of the isolated compounds
79.5 ± 1.1
86.5 ± 0.6
75.7 ± 3.1
77.3 ± 0.8
54.4 ± 3.4
42.7 ± 7.6
93.4 ± 2.2
83.9 ± 0.6
62.0 ± 2.2
94.6 ± 1.5
84.9 ± 1.2
0.67 ± 0.0
24.5 ± 0.2
In this study, scopolamine significantly reduced spontaneous alternation (%) in Y-maze test and time exploring the new object in object recognition test in scop 1.5 mg group mice. These indicated that scopolamine induces impairment of short-term spatial and non-spatial working memory. In Morris water maze test, scopolamine impaired gradual decrease of escape latencies, swimming distances during training session and reduced the time spent in target quadrant during probe session. These observations suggest that scopolamine not only impairs the process of acquisition by producing anterograde amnesia, which subsequently affects the retrieval of these. Morris water maze test represents the model of memory especially spatial memory. During the training trials, mouse locates the hidden platform using spatial cues. This model is very helpful to analyze the reversal amnesic effect with investigational drug because receptive trials with ongoing trials confirm the progress of reversal of amnesia [27–29].
In our experiment, administration of MEXX plus scopolamine-treated groups showed significantly shorter mean escape latencies and swimming distances than did the scopolamine-treated group in training session. The swimming time of the scopolamine-treated mice within the platform quadrant was significantly reduced by treating with MEXX (100 mg/kg) in probe session. This indicated that MEXX is able to protect mice from scopolamine-induced learning and memory (both acquisition and retrieval process) impairment as assessed by the Morris water maze test. The in vitro inhibitory activity on AChE and BChE of MEXX suggesting that the in vivo memory enhancing effect of MEXX due to its AChE inhibition in cells and tissues. The results are in correlations with those of previous studies on the effect of memory enhancing of some natural product such as: Black Maca, imperatorin, Lycium barbarum polysaccharides [27, 30–32].
Working memory is one of the short-term memories that could be impaired at an early stage of AD [2, 29]. Previous reports have shown that Y-maze test is the experimental paradigms appropriate to evaluate anti-dementia activities of drugs including natural products [29, 33]. Some plants exhibit the inhibitory activity on AChE reduced spontaneous alternation (%) in Y-maze test [27, 34]. In our experiment, we employed Y-maze test to investigate effect of MEXX in short-term spatial working memory. The experimental results showed MEXX (100 mg/kg) improved scopolamine-induced decrease in spontaneous alternation (%) while it did not affect in spontaneous locomotors. This suggests that MEXX alleviated the memory impairment induced by scopolamine injection.
The effect of the MEXX on cognitive impairment was further confirmed by using object recognition test . According to the results, no significant difference in total time spent exploring two identical objects was observed between control and scop 1.5 mg groups in sample phase session, indicating no differences in ability to recognize objects between animals. In the test phase session, the results showed that mice in the control group spent more time exploring the new object, whereas the scopolamine-treated mice showed no total time difference between familiar and new objects, indicating impairment of non-spatial object recognition memory. Administration of MEXX (100 mg/kg, p.o.) could significantly ameliorate scopolamin-induced recognition impairment against the new objects. This result is in correlation with other studies on Ptychopetalum olacoides , Acanthopanax trifoliatus , Lycium barbarum . These plants inhibited AChE activity and improved performance in object recognition test in scopolamine treated mice.
Previous authors indicated that performance in Y-maze, object recognition task are impaired by anti-cholinergic drugs, as well as cholinergic neuronal lesions [32, 37, 38]. Conversely, improved performance in Y maze, object recognition was observed with drugs that enhance cholinergic activity, and inhibit AChE [27, 30]. Alzheimer’s treatment drug such as piracetam and pramiracetam, were shown to improve learning, memory and cognition in Morris water maze, Y-maze and object recognition test . Our results are consistent with the notion that acetylcholine is critical in the processes underlying attention, learning and memory, the aging brain [3, 4].
General experimental procedures
The UV spectra were obtained with a Shimadzu UV-1800 recording spectrophotometer. The IR spectra were measured with a Shimadzu IR-408 spectrophotometer in CHCl3 solutions. The NMR spectra were taken on a Bruker Avance III 500 MHz spectrometer (Bruker Biospin) with tetramethylsilane (TMS) as an internal standard, and chemical shifts are expressed in δ values. The HR-ESI-MS was performed on a MicrO-QIITOF mass spectrometer (Bruker Daltonics). The ChE inhibitory reactions were recorded on 96-well microplates using a microplate reader (VersaMax ELISA, USA). Silica gel 60, 0.06–0.2 mm (70–230 mesh ASTM), for column chromatography was purchased from Scharlau (Barcelona, Spain). LiChroprep® RP-18 (40–63 μm) for liquid chromatography was purchased from Merck KGaA (Germany). Analytical and preparative TLC were carried out on precoated Merck Kieselgel 60F254 or RP-18F254 plates (0.25 or 0.5 mm thickness).
Animals and chemicals
Male Swiss mice (age, 8 weeks; weight, 25–27 g) were purchased from Military Medical University (Hanoi, Vietnam) and housed in a regulated environment (21 ± 2 °C, 12 h light/dark cycle, light period starting at 7 AM) with free access to food and water. Acetylcholinesterase (AChE) (EC 188.8.131.52), butyrylcholinesterase (BChE) (EC 184.108.40.206) and scopolamine hydrobromide (>98 %) were obtained from Sigma-Aldrich Pte Ltd (Nucleos, Singapore). Dithiobisnitrobenzoate (>99 %), berberine (>95 %) and DMSO were purchased from Merck (Darmstadt, Germany). Other chemicals were of the highest grade available.
The wood of X. xylocarpa was collected in Dak Lak province, Vietnam, in February 2012 and was identified by Dr. Truong LH, Southern Institute of Ecology, Vietnam Academy of Science and Technology. A voucher sample of the wood (P0046) has been deposited at the Department of Analytical Chemistry, Faculty of Chemistry, University of Science, Vietnam National University-Hochiminh City.
Extraction and isolation
Dried wood (9.0 kg) of X. xylocarpa was extracted with MeOH (15 L, reflux, 3 h × 3) to yield 480 g of methanolic extract (MEXX). The MeOH extract was suspended in H2O and partitioned successively with hexane, EtOAc, and BuOH to yield hexane (21 g), EtOAc (53 g), BuOH (180 g), and remaining aqueous (226 g) fractions, respectively. The EtOAc fraction (53 g) was subjected to silica gel column chromatography (10 × 120 cm), eluted with MeOH/CHCl3 (0–50 %) yielding thirteen fractions (fr.A, 0.4 g; fr.B, 0.5 g; fr.C, 0.9 g; fr.D, 7.8 g; fr.E, 2.1 g; fr.F, 3.2 g; fr.G, 1.9 g; fr.H, 1.9 g; fr.I, 1.2 g; fr.J, 0.3 g; fr.K, 4.1 g; fr.L, 7.8 g and fr.M, 20.5 g). Fraction fr.B (0.5 g) was applied to silica gel column chromatography (2 × 80 cm), eluted with EtOAc/hexane (0–80 %) to give four subfractions (fr.B1–B5). Subfractions fr.B2 and fr.B3 were rechromatographed on a silica gel column with EtOAc/hexane as eluent to give compounds 2 (17.6 mg), and 12 (2.4 mg). Fraction fr.C (0.7 g) was also subjected to silica gel column chromatography (2 × 80 cm), eluted with EtOAc/hexane (0–80 %) to afford three subfractions (fr.C1–C3). Subfraction fr.C1 was separated by column chromatography with EtOAc/hexane as eluent (0–60 %) and purified by preparative TLC to obtain 5 (3.5 mg) and 6 (6.3 mg). Subfraction fr.C2 was further separated by silica gel column chromatography, eluted with EtOAc/hexane and CHCl3/hexane to give compound 11 (2.6 mg). Fraction fr.D (7.8 g) was dissolved in CHCl3/hexane (20:80) to gain the precipitation of 10 (2.4 g), the remaining part was further separated by silica gel column chromatography (5 × 80 cm) with EtOAc/hexane (0–80 %) to yield four subfractions (fr.D1–D4). Subfraction fr.D1 was rechromatographed on silica gel column chromatography with EtOAc/hexane to give 3 (2.8 mg) and 8 (3.1 mg). Subfraction fr.D3 was subjected to silica gel column chromatography and successively eluted with acetone/hexane (0–80 %), EtOAc/CHCl3 (0–50 %), acetone/CHCl3 (0–80 %), and then followed by preparative TLC with acetone/hexane (8:92) to afford 1 (15.7 mg), 4 (19.3 mg) and 9 (3.2 mg). Fraction fr.E (2.1 g) was separated by silica gel column chromatography (3 × 80 cm) with MeOH/CHCl3 (0–30 %) as eluent to yield four subfractions (fr.E1–E4). Subfractions fr.E1 and fr.E2 were purified by preparative TLC with EtOAc/hexane (20:80) and acetone/hexane (6:94) to yield 13 (14.6 mg), 14 (5.8 mg) and 15 (10.2 mg). Subfraction fr.E3 was further separated by silica gel column chromatography with MeOH/CHCl3 to give four subfraction (fr.E3.1–E3.4). Subfraction fr.E3.1 was rechromatographed on a silica gel column with EtOAc/hexane as eluent (0–60 %) to afford compound 16 (5.6 mg). The insoluble subfraction fr.E3.4 was dissolved in acetone/hexane (10:90) and recrystallized to yield 18 (15.9 mg). Fraction fr.F (3.2 g) was further separated by silica gel column chromatography (3 × 80 cm) eluted with EtOAc/hexane (0–50 %) and MeOH/CHCl3 (0–30 %) and to give 19 (3.6 mg). Fraction fr.G (1.9 g) was subjected to silica gel column chromatography (3 × 80 cm) eluted with MeOH/CHCl3 (0–60 %) to give four subfractions (fr.G1–G4). Subfraction fr.G1 and fr.G2 was rechromatographed on silica gel column with EtOAc/hexane and CHCl3/hexane and respectively purified by preparative TLC with acetone/CHCl3 (10:90) and MeOH/CHCl3 (10:90) to give 7 (156.3 mg) and 21 (3.5 mg), respectively. Compound 19 (160 mg) was recrystallised from the insoluble fraction of fr.I (1.2 g) in acetone/hexane (10:90), and the remaining part was applied to silica gel column chromatography (2 × 80 cm) with MeOH/CHCl3 as eluent (0–50 %), the eluate was concentrated and crystallised in acetone/hexane (10:90) to afford 17 (10.2 mg).
(3β)-Hopan-3-ol-28,22-olide ( 1 ): White amophous powder, IR (CHCl3) cm−1: 3310, 1730, 1170, 1100. 1H-NMR (CDCl3, 500 MHz) and 13C-NMR (CDCl3, 125 MHz), see Table 1. HR-ESI-MS m/z: 457.3674 [M + H]+ and 479.3482 [M + Na]+ (Calcd for C30H49O3, 457.3682; C30H48O3Na, 479.3501) (for further information, see Additional file 1).
AChE and BChE inhibition assay
The inhibitory activities of the ChEs were measured using a modified Ellman’s method . Acetylthiocholine and butyrylthiocholine were used as substrates to examine the inhibitory effect of sample on the AChE and BChE action, respectively. The reaction mixture contained: 140 μL of sodium phosphate buffer (pH 8.0); 20 μL of tested sample solution; and 20 µL of either AChE or BChE solution (5 units/mL), which were mixed and incubated at room temperature for 15 min. The reactions were initiated by the addition of 10 µL of dithiobisnitrobenzoate (DTNB) and 10 μL of either acetylthiocholine or butyrylthiocholine, respectively. The hydrolysis of AChE or BChE was monitored at 412 nm based on the formation of yellow 5-thio-2-nitrobenzoate anion from the reaction of DTNB with thiocholine, which was released by enzymatic hydrolysis of either AChE or BChE. All tested samples and the positive control, berberine , were dissolved in 10 % DMSO (analytical grade). The reaction was performed in triplicate and recorded in 96-well microplates using a microplate reader (VersaMax ELISA, USA). Percent inhibition was calculated from (1–S/E) × 100, where E and S were the enzyme activities with and without the tested sample, respectively. The ChE inhibitory activity of each sample was expressed in terms of the IC50 value (μM required to inhibit the hydrolysis of the substrate, AChE or BChE, by 50 %), as calculated from the logarithmic dose-inhibition curve.
Animal grouping and drug treatment
The male Swiss mice were randomly assigned to five treatment groups (n = 12 per group): (1) Control (Saline), (2) Scop 1.5 mg (scopolamine 1.5 mg/kg/day), (3) XX 50 mg (MEXX 50 mg/kg/day + scopolamine 1.5 mg/kg/day), (4) XX 75 mg (MEXX 75 mg/kg/day + scopolamine 1.5 mg/kg/day) and (5) XX 100 mg (MEXX 100 mg/kg/day + scopolamine 1.5 mg/kg/day). MEXX was dissolved in saline and administered by oral gavage (p.o.). Scopolamine was also dissolved in saline and administered by intraperitoneal (i.p.) injection. MEXX was administered 60 min before the trial, and scopolamine was injected 30 min before the trial.
Morris water Y-maze test
The Morris water maze is a black circular pool (80 cm in diameter and 35 cm in height) with a featureless inner surface. The circular pool was filled with water and nontoxic water-soluble black dye (20 ± 1 °C). The pool was divided into four quadrants of equal area. A transparent platform (4 cm in diameter and 18 cm in height) was centered in one of the four quadrants of the pool and submerged 1 cm below the water surface so that it was invisible at water level. The pool was located in a test room, which contained various prominent visual cues. The position of platform for escape and the visual cues remained unchanged throughout the experiments. The location of each swimming mouse, from the start position to the platform, was monitored by a video tracking system (ANY-maze, Stoelting, USA). In the water maze experiments, the day prior to the experiment was dedicated to swim training for 60 s in the absence of the platform. During the seven subsequent days, the mice were given four training-trials per session per day and an inter-trial interval of 2 min. For each training-trial, mice were placed in the water facing the pool wall in a randomly selected pool quadrant, the escape latencies and distance swim were recorded. These parameters were averaged for each day and for each mouse. Once the mouse located the platform, it was permitted to remain on it for 10 s. If the mouse did not locate the platform within 60 s, it was placed on the platform for 10 s and then removed from the. On day 8, the probe test involved removing the platform from the pool. That test was performed with the cut-off time of 120 s. The point of entry of the mouse into the pool was changed each trial thereafter. Mice were treated with saline or MEXX (50, 75 or 100 mg/kg, p.o.) given before the training trial. After 30 min, amnesia was induced in mice with scopolamine (1.5 mg/kg) given intraperitoneal injection. All mice were tested for spatial memory 30 min after scopolamine treatment.
The Y-maze is a three-arm maze with equal angles between all arms, which were 35 cm length and 5 cm width, with walls 10 cm high. The maze floor and walls were constructed from dark grey polyvinyl plastic. Mice were initially placed within one arm, and the sequence and number of arm entries were recorded 10-min period for each mouse and analyzed monitored by a video tracking system (ANY-maze, Stoelting, USA). One hour before the test, mice in control group and scop 1.5 mg group received distilled water and other mice were administered MEXX (50, 75, or 100 mg/kg, p.o.). After 30 min, memory impairment was induced by administering scopolamine (1.5 mg/kg, i.p.). Arms were cleaned between tests to remove odors and residues by diluted 10 % ethanol. Alternation behavior was determined from successive entries into three different arms (e.g., ABC, CAB, or BCA). An arm entry by the mice was defined as placing all four paws within a boundary of the arm. The alternation score (%) for each mouse was defined as the ratio of the actual number of alternations to the possible number (defined as the total number of arm entries minus two) multiplied by 100 as shown by the following equation: % Alternation = [(Number of alternations)/(Total arm entries − 2)] × 100. The number of arm entries was used as an indicator of locomotor activity.
Object recognition test
The task took place in a to an open-field box (45 × 45 × 50 cm). Firstly, all animals were submitted to a habituation session, freely exploring the object free open field for 5 min. Twenty-four hours later, the sample phase session took place by placing individual mice for 5 min at the field in which two identical objects (A1 and A2; identical toys) were placed in a symmetrical position about 10 cm away from the wall; exploration was defined as the time spent sniffing or touching the object with the nose and/or forepaws. Test phase session were performed 24 h after training, when mice were allowed to explore the open field for 5 min in the presence of one familiar (A) and one novel (B) object. One hour before test phase session, mice were administered MEXX (50, 75, or 100 mg/kg, p.o.). The control group received distilled water. After 30 min, memory impairment was induced by administering scopolamine (1.5 mg/kg, i.p.). All objects presented similar textures and sizes, but distinctive shapes; after each trial objects were washed with 10 % ethanol to discard smells or residues. The exploration time and frequencies were recorded, n = 12 per group.
The results of the behavioral studies are expressed as mean ± SEM, Y-maze test spontaneous alternation (%), object recognition test distance travel and Morris water maze test probe-trial swimming times were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc for multiple comparisons. The object recognition test time spent exploring a familiar and novel object in sample and test phase were analyzed by pair t-test. The Morris water maze test training-trial escape latencies and distance were analyzed by two-way ANOVA repeated followed by Tukey’s post hoc analysis using the day as one variable and treatment as a second. Statistical significance was set at P < 0.05.
In conclusion, a new hopan-type triterpenoid, (3β)-hopan-3-ol-28,22-olide (1) was isolated together with twenty known compounds (2–21). Compound 1, 2, 4, 5, 7–9, and 11–13 exhibited potent acetylcholinesterase (AChE); and compound 13 was also shown anti-butyrylcholinesterase (BChE) activity. The cognitive-enhancing effect of the MEXX on amnesic mice induced by scopolamine in vivo. It is noteworthy that MEXX is effective when administered orally to mice, experimental results are consistent with the traditional use of this medicinal plant species, the data here reported justify further studies with this plant extract in the context of treating attention and cognitive deficits associated with neurodegenerative diseases.
LMTL, MTTN, NTN and MVC designed research; LMTL, HXN, HTN, NMN, BSM, JAK, and JSC performed research; LMTL, PHD and NTN analyzed spectral data; TMH, HXN, HTN, and NMN and MVC analyzed biological data; LMTL, MVC and MTTN wrote the paper. All authors read and approved the final manuscript.
This work was supported by Grant 106-YS.05-2013.24 from Vietnam’s National Foundation for Science and Technology Development (NAFOSTED).
The authors declare that they have no competing interests.
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- Blennow K, de Leon MJ, Zetterberg H (2006) Alzheimer’s disease. Lancet 368:387–403View ArticleGoogle Scholar
- Cummings JL (2004) Alzheimer’s disease. N Engl J Med 351:56–67View ArticleGoogle Scholar
- McGleenon BM, Dynan KB, Passmore AP (1999) Acetylcholinesterase inhibitors in Alzheimer’s disease. Br J Clin Pharmacol 48:471–480View ArticleGoogle Scholar
- Garcia-Ayllon MS, Small DH, Avila J, Saez-Valero J (2011) Revisiting the role of acetylcholinesterase in Alzheimer’s disease: cross-talk with P-tau and beta-amyloid. Front Mol Neurosci 4:1–9View ArticleGoogle Scholar
- Vo VC (2011) Dictionary of Vietnamese medicinal plants. Medicine Publisher, HanoiGoogle Scholar
- Nakmee PS, Khuntong S, Nuengchamnong N (2016) Phytochemical constituents with antimicrobial and antioxidant activities from Xylia xylocarpa (Roxb.) Taub. Sawdust extracts. Chiang Mai J Sci 43:11–21Google Scholar
- Burns D, Reynolds WF, Buchanan G, Reese PB, Enriquez RG (2000) Assignment of 1H and 13C spectra and investigation of hindered side-chain rotation in lupeol derivatives. Magn Reson Chem 38:488–493View ArticleGoogle Scholar
- Lee CK (1998) A new norlupene from the leaves of Melaleuca leucadendron. J Nat Prod 61:375–376View ArticleGoogle Scholar
- Tijjani A, Ndukwe IG, Ayo RG (2012) Isolation and characterization of lup-20(29)-ene-3,28-diol (Betulin) from the stem-bark of Adenium obesum (Apocynaceae). Trop J Pharm Res 11:259–262View ArticleGoogle Scholar
- Abdel Bar FM, Zaghloul AM, Bachawal SV, Sylvester PW, Ahmad KF, El Sayed KA (2008) Antiproliferative triterpenes from Melaleuca ericifolia. J Nat Prod 71:1787–1790View ArticleGoogle Scholar
- Hassan RA, Hassan EM, Brahim NA, Nazif NM (2015) Triterpenes and cytotoxic activity of Acokanthera oblongifolia Hochst. growing in Egypt. Res J Pharm Biol Chem Sci 6:1677–1686Google Scholar
- Satiraphan M, Pamonsinlapatham P, Sotanaphun U, Sittisombut C, Raynaud F, Garbay C, Michel S, Cachet X (2012) Lupane triterpenes from the leaves of the tropical rain forest tree Hopea odorata Roxb. and their cytotoxic activities. Biochem Syst Ecol 44:4073–4412View ArticleGoogle Scholar
- Maillard M, Adewunmi CO, Hostettmann K (1992) A triterpene glycoside from the fruits of Tetrapleura tetraptera. Phytochemistry 31:1321–1323View ArticleGoogle Scholar
- Salvador JA, Pinto RM, Santos RC, Le Roux C, Beja AM, Paixao JA (2009) Bismuth triflate-catalyzed Wagner-Meerwein rearrangement in terpenes. Application to the synthesis of the 18alpha-oleanane core and A-neo-18alpha-oleanene compounds from lupanes. Org Biomol Chem 7:508–517View ArticleGoogle Scholar
- Achari B, Pakrashi SC (1976) Studies on Indian medicinal plants—XXXIX: reinvestigation of the lactones and bromo derivative of betulinic acid. Tetrahedron 32:741–744View ArticleGoogle Scholar
- Cui XR, Tsukada M, Suzuki N, Shimamura T, Gao L, Koyanagi J, Komada F, Saito S (2008) Comparison of the cytotoxic activities of naturally occurring hydroxyanthraquinones and hydroxynaphthoquinones. Eur J Med Chem 43:1206–1215View ArticleGoogle Scholar
- Peng W, Han T, Liu Q, Qin L (2011) Chemical constituents from aerial part of Atractylodes macrocephala. Zhongguo Zhongyao Zasshi 36:578–581Google Scholar
- Prachayasittikul S, Suphapong S, Worachartcheewan A, Lawung R, Ruchirawat S, Prachayasittikul V (2009) Bioactive metabolites from Spilanthes acmella Murr. Molecules 14:850–867View ArticleGoogle Scholar
- Galland S, Mora N, Abert-Vian M, Rakotomanomana N, Dangles O (2007) Chemical synthesis of hydroxycinnamic acid glucosides and evaluation of their ability to stabilize natural colors via anthocyanin copigmentation. J Agric Food Chem 55:7573–7579View ArticleGoogle Scholar
- X-h Cai, Xie B, Guo H (2006) Synthesis and evaluation of methyl 2-methoxycarbonyl-3-phenylpropionate derivatives as a new type of angiotensin converting enzyme inhibitors. Can J Chem 84:1110–1113View ArticleGoogle Scholar
- Lee H, Lee H, Lee K, Kang H, Lee D, Khan M (2010) Triterpene saponins and other constituents from Fatsia japonica. Chem Nat Compd 46:499–501View ArticleGoogle Scholar
- Chatsumpun M, Sritularak B, Likhitwitayawuid K (2010) Phenolic compounds from stem wood of Millettia leucantha. Chem Nat Compd 46:634–635View ArticleGoogle Scholar
- Pouységu L, Sylla T, Garnier T, Rojas LB, Charris J, Deffieux D, Quideau S (2010) Hypervalent iodine-mediated oxygenative phenol dearomatization reactions. Tetrahedron 66:5908–5917View ArticleGoogle Scholar
- Li-qin W, Rong H, Fu-chi Z, Qiu-xiang F, Ye-gao C (2011) Chemical constituents of Combretum punctatum spp. squamosum. Chem Nat Compd 47:470–471View ArticleGoogle Scholar
- Nakane T, Arai Y, Masuda K, Ishizaki Y, Ageta H, Shiojima K (1999) Fern constituents: six new triterpenoid alcohols from Adiantum capillus-veneris. Chem Pharm Bull 47:543–547View ArticleGoogle Scholar
- Kamaya R, Mori T, Shoji K, Ageta H, Chang H, Hsu H (1991) Fern constituents: triterpenoids from Oleandra wallichii. Yakugaku Zasshi 111:120–125Google Scholar
- Rubio J, Dang H, Gong M, Liu X, Chen SL, Gonzales GF (2007) Aqueous and hydroalcoholic extracts of Black Maca (Lepidium meyenii) improve scopolamine-induced memory impairment in mice. Food Chem Toxicol 45:1882–1890View ArticleGoogle Scholar
- Vorhees CV, Williams MT (2006) Morris water maze: procedures for assessing spatial and related forms of learning and memory. Nat Protoc 1:848–858View ArticleGoogle Scholar
- Whishaw IQ, Kolb B (2004) The behavior of the laboratory rat. Oxford University Press, OxfordView ArticleGoogle Scholar
- Budzynska B, Boguszewska-Czubara A, Kruk-Slomka M, Skalicka-Wozniak K, Michalak A, Musik I, Biala G (2015) Effects of imperatorin on scopolamine-induced cognitive impairment and oxidative stress in mice. Psychopharmacology 232:931–942View ArticleGoogle Scholar
- Chen W, Cheng X, Chen J, Yi X, Nie D, Sun X, Qin J, Tian M, Jin G, Zhang X (2014) Lycium barbarum polysaccharides prevent memory and neurogenesis impairments in scopolamine-treated rats. PLoS ONE 9:1–13Google Scholar
- Ennaceur A, Meliani K (1992) Effects of physostigmine and scopolamine on rats’ performances in object-recognition and radial-maze tests. Psychopharmacology 109:321–330View ArticleGoogle Scholar
- Gotz J, Ittner LM (2008) Animal models of Alzheimer’s disease and frontotemporal dementia. Nat Rev Neurosci 9:532–544View ArticleGoogle Scholar
- Kwon SH, Kim HC, Lee SY, Jang CG (2009) Loganin improves learning and memory impairments induced by scopolamine in mice. Eur J Pharmacol 619:44–49View ArticleGoogle Scholar
- Antunes M, Biala G (2012) The novel object recognition memory: neurobiology, test procedure, and its modifications. Cogn Process 13:93–110View ArticleGoogle Scholar
- Sithisarn P, Rojsanga P, Jarikasem S, Tanaka K, Matsumoto K (2013) Ameliorative effects of Acanthopanax trifoliatus on cognitive and emotional deficits in olfactory bulbectomized mice: an animal model of depression and cognitive deficits. Evid Based Complement Alternat Med 2013:1–10View ArticleGoogle Scholar
- Wirths O, Bayer TA (2010) Neuron loss in transgenic mouse models of Alzheimer’s disease. Int J Alzheimers Dis 2010:1–6View ArticleGoogle Scholar
- Bartolini L, Casamenti F, Pepeu G (1996) Aniracetam restores object recognition impaired by age, scopolamine, and nucleus basalis lesions. Pharmacol Biochem Behav 53:277–283View ArticleGoogle Scholar
- Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95View ArticleGoogle Scholar
- Lee I, Ahn B, Choi J, Hattori M, Min B, Bae K (2011) Selective cholinesterase inhibition by lanostane triterpenes from fruiting bodies of Ganoderma lucidum. Bioorg Med Chem Lett 21:6603–6607View ArticleGoogle Scholar