Tyrosinase inhibitory activity of flavonoids from Artocarpus heterophyllous
© Nguyen et al. 2016
Received: 30 October 2015
Accepted: 10 January 2016
Published: 29 January 2016
Tyrosinase is an oxidoreductase that is very important in medicine and cosmetics because the excessive production of melanin causes hyperpigmentation. The development of novel, effective tyrosinase inhibitors has long been pursued. In preliminary tests, we found that an extract of the wood of Artocarpus heterophyllous (AH) potently inhibited tyrosinase activity.
Two new flavonoids, artocaepin E (1) and artocaepin F (2), were isolated from the wood of AH, together with norartocarpetin (3), artocarpanone (4), liquiritigenin (5), steppogenin (6), and dihydromorin (7). Their structures were elucidated using one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) and mass spectrometry. The absolute configuration of 2 was determined from the circular dichroism (CD) spectrum. Artocarpanone (4) had the most potent tyrosinase inhibitory effect, with an IC50 of 2.0 ± 0.1 μM, followed by artocaepin E (1) and steppogenin (6), with IC50 values of 6.7 ± 0.8 and 7.5 ± 0.5 μM, respectively. A kinetic investigation indicated that 1 showed competitive inhibition, with an inhibition constant (K i) of 6.23 μM.
Tyrosinase is a key enzyme in mammalian melanin synthesis. It catalyzes the first step in two reactions of melanin synthesis: the hydroxylation of l-tyrosine to 3,4–dihydroxyphenylalanine (l-DOPA) and the oxidation of l-DOPA to dopaquinone. This o-quinone is a highly reactive compound that can polymerize spontaneously to form melanin . In mammals, melanin protects the skin from ultraviolet (UV) damage by absorbing UV sunlight and removing reactive oxygen species. However, the production of abnormal melanin pigmentation is an esthetic problem in humans . Therefore, the development of high-performance tyrosinase inhibitors is much needed.
Artocarpus heterophyllous Lam belongs to the family Moraceae and is popularly known as jackfruit; it is distributed widely in tropical and subtropical regions of Asia. In Vietnam, this plant is known as “Mit,” and is cultivated for its edible fruits, while the wood has been used for its anti-inflammatory, antioxidant, and antiaging effects . In preliminary tests, we found that an extract of the wood of AH strongly inhibited tyrosinase activity. This plant is a rich source of prenylated flavonoids and their derivatives. Numerous compounds have been isolated from AH, including prenylated flavonoids, chalcones, and simple polyphenols. These possess various biological activities, including cytotoxic, tyrosinase inhibitory, anti-inflammatory, and antioxidant effects [4–6]. The present phytochemical investigation of the wood of AH led to the isolation of two new flavonoids (1, 2) and five known compounds (3−7). In this paper, we report the isolation and structure elucidation of these isolated compounds, as well as their tyrosinase inhibitory activities.
Results and discussion
1H (500 MHz, J in Hz) and 13C (125 MHz) NMR data for 1 and 2
5.44 dd (12.8; 3.0)
2.70 dd (16.7; 3.0)3.00 dd (16.7; 12.8)
7.59 d (8.6)
6.63 d (8.6)
7.42 d (8.6)
6.49 d (2.4)
6.90 d (8.6)
6.44 dd (8.8; 2.4)
6.90 d (8.6)
7.73 d (8.8)
7.42 d (8.6)
3.30 d (7.2)
3.38 d (7.3)
5.55 t (7.2)
5.49 t (7.3)
7.53 d (8.7)
6.78 d (8.7)
7.54 d (16.0)
6.39 d (16.0)
Artocaepin F (2) was isolated as a yellow amorphous solid. The molecular formula of 2 was determined to be C20H20O5 by HR-ESI–MS. The IR spectrum showed absorption bands of hydroxy (3400 cm−1), phenyl (1600, 1450 cm−1), and ketone (1705 cm−1) groups. The 1H NMR spectra of 2 (Table 1) showed signals for two sets of ortho-coupled aromatic protons at δ H 7.42 (2H, d, J = 8.6 Hz, H-2′, and H-6′) and 6.90 (2H, d, J = 8.6 Hz, H-3′ and H-5′), and others at δ 7.59 (1H, d, J = 8.6 Hz, H-5) and 6.63 (1H, d, J = 8.6 Hz, H-6), together with two methylene signals at δ H 2.70 (1H, dd, J = 16.7, 3.0 Hz, H-3a) and 3.00 (1H, dd, J = 16.7, 12.8 Hz, H-3b), and one oxymethine at δ H 5.44 (1H, dd, J = 12.8, 3.0 Hz, H-2), which are typical of the flavanone skeleton . The 13C NMR and DEPT spectrum of 2 displayed 20 carbon signals, including 15 carbon signals due to the flavanone skeleton and five belonging to a 4-hydroxyprenyl moiety (Fig. 1). The 4-hydroxyprenyl group was located at C-8 based on the HMBC correlations between H-1″ (δ H 3.38, 2H, d, J = 7.3 Hz) and C-7, C-8, C-8a, and of H-2″ (δ H 5.49, 1H, t, J = 7.3 Hz) with C-8 (Fig. 2). The NOESY correlations of H-1″ with H-5″ and of H-2″ with H-4″ indicated that the configuration of the C-2″/C-3″ double bond of 2 was the same as that of 1. Finally, the absolute configuration at C-2 was considered to be S according to the results of the CD spectroscopic analysis, which showed negative and positive Cotton effects at 290 and 334 nm, respectively . From this spectroscopic evidence, the structure of artocaepin F was concluded to be 2.
Tyrosinase inhibitory activity of the isolated compounds 1−7
6.7 ± 0.8
2.0 ± 0.1
22.0 ± 2.5
7.5 ± 0.5
44.6 ± 0.4
On close inspection of the inhibitory activity exerted by these compounds, the following biological profile of the structure–activity relationship was deduced. In terms of the flavone skeleton, compounds 1 and 3 are derivative of apigenin, a common flavone in plants; however, the presence of one hydroxyl group at C-2′, a trans-p-coumaroyl unit connected to the hydroxyprenyl through an ester linkage at C-6 of the apigenin skeleton in 1, led to significantly stronger inhibitory activity than that of 3 (6.7 vs. >50 μM). This suggests that the absence of the side-chain at C-6 of the B-ring leads to a significant loss of activity, and the presence of a side-chain such as trans-p-coumaroyl connected to the hydroxyprenyl may positively influence the tyrosinase inhibitory activity. Regarding the flavanone skeleton, artocarpanone (4), which possesses a methoxyl group at C-7 of ring A, had the strongest inhibitory activity. Steppogenin (6) shares the same structure as 4, except for the hydroxyl group at C-7; however, it had 3.75-fold higher inhibitory activity than 4. In comparison, dihydromorin (7), which has four hydroxyl groups at C-2′, C-3, C-5, and C-7, had weak activity. These results imply that the methoxyl and hydroxyl groups in the main flavanone skeleton play an important role in tyrosinase inhibition.
Optical rotations were recorded on a JASCO DIP-140 digital polarimeter. CD measurements were carried out on a JASCO J-805 spectropolarimeter. IR spectra were measured with a Shimadzu IR-408 spectrophotometer in CHCl3 solution. NMR spectra were taken on a Bruker Advance III 500 spectrometer (Brucker Biospin) with tetramethylsilane (TMS) as an internal standard, and chemical shifts are expressed in δ values. HR-ESI–MS measurements were carried out on a Bruker microTOF-QII spectrometer. Column chromatography was performed with BW-820MH Si gel (Fuji Silisia, Aichi, Japan). Analytical and preparative TLC was carried out on precoated Merk Kiesegel 60F254 or RP-18F254 plates (0.25 or 0.5 mm thickness).
Tyrosinase (EC 220.127.116.11) from mushroom (3933 U/mL) and l-dihydroxyphenylalanine (l-DOPA) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Kojic acid and DMSO were purchased from Merck (Darmstadt, Germany). Other chemicals were of the highest grade available.
The wood of A. heterophyllous was collected at the Seven-Mountain area, An Giang province, Vietnam in August 2010. The plant was identified by Ms. Hoang Viet, Faculty of Biology, University of Science, Vietnam National University-Hochiminh City. The voucher sample of the wood part (AN-2985) is preserved at Department of Analytical Chemistry, Faculty of Chemistry, University of Science, Vietnam National University-Hochiminh City.
Extraction and isolation
The dried powder of wood of A. heterophyllous (5.8 kg) was extracted with MeOH (15 L, reflux, 3 h, × 3) to yield a MeOH extract. The extract was partitioned between EtOAc and water to give an EtOAc-soluble fraction (64.2 g). The EtOAc-soluble fraction was subjected to silica gel column chromatography with acetone−hexane to give six fractions fr. 1–6. Fraction 6 was chromatographed further using a MeOH−CHCl3 gradient system to afford four subfractions fr. 6.1–6.4. Sub-fraction 6.2 was chromatographed further using MeOH−CHCl3 gradient system, with final purification effected by preparative TLC with 2 % MeOH−CHCl3, to give 4 (6.5 mg) and 5 (20.8 mg). Subfraction 6.3 was separated by preparative TLC with 5 % MeOH−CHCl3 to give 1 (5.0 mg), and 2 (5.3 mg), and 3 (8.5 mg). Subfraction 6.4 was re-chromatographed on silica gel with 7 % MeOH−CHCl3, followed by final purification using preparative TLC with 40 % acetone−hexane, to give 6 (8.0 mg), and 7 (7.5 mg).
Artocaepin E (1): pale yellow, amorphous solid; IR ν max (CHCl3) 3395, 1655, 1615, 1400 cm−1; 1H and 13C NMR (DMSO-d 6 500 MHz) see Table 1; HR-ESI–MS m/z 517.1487 (calcd. for C29H25O9 [M + H]+, 517.1499).
Artocaepin F (2): yellowish gum; [α] D 25 −10.0° (c 1.0, C2H5OH); IR ν max (CHCl3) 3365, 1630, 1600, 1510 cm−1; 1H and 13C NMR (acetone-d 6 500 MHz) see Table 1; HR-ESI–MS m/z 363.1224 (calcd. for C20H20O5Na [M + Na]+, 363.1208).
Tyrosinase inhibitory assay
All the samples were first dissolved in DMSO and used for the actual experiment at concentrations of 100-1 µg/mL (or µM for pure compounds). The tyrosinase inhibitory activity assay was performed as previously described by Arung et al. . The assay mixtures consisting of 1900 µL of test solution in 0.1 M phosphate buffer pH 6.8 and 100 µL of enzyme solution (15 U/mL in 0.1 M phosphate buffer pH 6.8) was prepared immediately before use. After preincubation at room temperature for 30 min, the reaction was initiated by the addition of 1000 µL of substrate solution (1.5 mM l-DOPA in 0.1 M phosphate buffer pH 6.8). The assay mixture was incubated at room temperature for 7 min, and the absorbance at 475 nm was measured with a Shimadzu UV-1800 spectrophotometer. Kojic acid, a known tyrosinase inhibitor, was used as positive control. Tyrosinase inhibitory activity was expressed as the percentage inhibitory of enzyme tyrosinase in the above assay system, calculated as (1 − B/A) × 100, where A and B are the activities of the enzyme without and with test material. IC50 values were calculated from the mean values of data from four determinations.
The procedure for determination of the inhibition mechanism was similar to that for determination of IC50, except that uninhibited and inhibited reactions were observed for three different concentrations of l-DOPA (0.2, 0.3, 0.4, 0.5, and 0.6 mM) at 30 °C in 0.1 M phosphate buffer pH 6.8. The dependence of absorbance (475 nm) on time was measured, and the reaction rate was calculated for all reactions (uninhibited and inhibited). Then, a Lineweaver–Burk plot was constructed, and K m and V m values were calculated. Each measurement was performed in duplicate.
In this study, we identified two new flavonoids from the wood of AH, artocaepin E (1) and artocaepin F (2), together with five known compounds: norartocarpetin (3), artocarpanone (4), liquiritigenin (5), steppogenin (6), and dihydromorin (7). Regarding tyrosinase inhibition, artocarpanone (4) had the greatest inhibitory effect, followed by artocaepin E (1) and steppogenin (6). Liquiritigenin (5) also showed significant concentration-dependent inhibition. Kinetic studies indicated that the new active compound artocaepin E (1) displayed competitive inhibition. These results suggest that these compounds may serve as structural templates for the design and development of novel tyrosinase inhibitors as effective anti-browning agents in cosmetics.
HXN and NTN isolated and elucidated the compounds, MHKN and THL carried out the bioassay, TNVD and TMH wrote the manuscript, MTTN carried out conception and design of the study, read and brought some corrections to the paper. All authors read and approved the final manuscript.
This research is funded by Vietnam National University Hochiminh City (VNU-HCM) under Grant number A2015-18-02.
The authors declare that they have no competing interests.
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- Seo SY, Sharma VK, Sharma N (2003) Mushroom tyrosinase: recent prospects. J Agric Food Chem 51:2837–2853View ArticleGoogle Scholar
- Briganti S, Camera E, Picardo M (2003) Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res 16:101–110View ArticleGoogle Scholar
- Vo.VC An giang medicinal plants. Sci Technol Publisher: An Giang, Vietnam. 1991.Google Scholar
- Septama AW, Panichayupakaranant P (2015) Antibacterial assay-guided isolation of active compounds from Artocarpus heterophyllous heartwoods. Pharm Biol 53:1608–1613View ArticleGoogle Scholar
- Ruiz-Montanez G, Burgos-Hernandez A, Calderon-Santoyo M, Lopez-Saiz CM, Velazquez-Contreras CA, Navarro-Ocana A et al (2015) Screening antimutagenic and antiproliferative properties of extracts isolated from Jackfruit pulp (Artocarpus heterophyllous Lam). Food Chem 175:409–416View ArticleGoogle Scholar
- Shrikanta A, Kumar A, Govindaswamy V (2015) Resveratrol content and antioxidant properties of underutilized fruits. J Food Sci Technol 52:383–390View ArticleGoogle Scholar
- Jeong SH, Ryu YB, Curtis-Long MJ, Ryu HW, Baek YS, Kang JE et al (2009) Tyrosinase inhibitory polyphenols from roots of Morus lhou. J Agric Food Chem 57:1195–1203View ArticleGoogle Scholar
- Wei BL, Weng JR, Chiu PH, Hung CF, Wang JP, Lin CN (2005) Anti-inflammatory flavonoids from Artocarpus heterophyllous and Artocarpus communis. J Agric Food Chem 53:3867–3871View ArticleGoogle Scholar
- Kulesh NI, Vasilevskaya NA, Veselova MV, Denisenko VA, Fedoreev SA (2008) Minor polyphenols from Maackia amurensis wood. Chem Nat Compd 44:712–714View ArticleGoogle Scholar
- Zheng ZP, Cheng KW, To JT, Li H, Wang M (2008) Isolation of tyrosinase inhibitors from Artocarpus heterophyllous and use of its extract as antibrowning agent. Mol Nutr Food Res 52:1530–1538View ArticleGoogle Scholar
- Nguyen NT, Nguyen MHK, Nguyen HX, Bui NKN, Nguyen MTT (2012) Tyrosinase inhibitors from the wood of Artocarpus heterophyllous. J Nat Prod 75:1951–1955View ArticleGoogle Scholar
- Slade D, Ferreira D, Marais JPJ (2005) Circular dichroism, a powerful tool for the assessment of absolute configuration of flavonoids. Phytochemistry 66:2177–2215View ArticleGoogle Scholar
- Arung ET, Kusuma IW, Iskandar YM, Yasutake S, Shimizu K, Kondo R (2005) Screening of Indonesian plants for tyrosinase inhibitory activity. J Wood Sci 51:520–525View ArticleGoogle Scholar