Mechanical, physico-chemical, and antimicrobial properties of gelatin-based film incorporated with catechin-lysozyme
© Rawdkuen et al.; licensee Chemistry Central Ltd. 2012
Received: 23 September 2012
Accepted: 23 October 2012
Published: 7 November 2012
Microbial activity is a primary cause of deterioration in many foods and is often responsible for reduced quality and safety. Food-borne illnesses associated with E. coli O157:H7, S. aureus, S. enteritidis and L. monocytogenes are a major public health concern throughout the world. A number of methods have been employed to control or prevent the growth of these microorganisms in food. Antimicrobial packaging is one of the most promising active packaging systems for effectively retarding the growth of food spoilage and pathogenic microorganisms. The aim of this study was to determine the mechanical, physico-chemical properties and inhibitory effects of the fish gelatin films against selected food spoilage microorganisms when incorporated with catechin-lysozyme.
The effect of the catechin-lysozyme combination addition (CLC: 0, 0.125, 0.25, and 0.5%, w/v) on fish gelatin film properties was monitored. At the level of 0.5% addition, the CLC showed the greatest elongation at break (EAB) at 143.17% with 0.039 mm thickness, and the lowest water vapor permeability (WVP) at 6.5 x 10−8 g·mm·h-1·cm-2·Pa-1, whereas the control showed high tensile strength (TS) and the highest WVP. Regarding color attributes, the gelatin film without CLC addition gave the highest lightness (L* 91.95) but lowest in redness (a*-1.29) and yellowness (b* 2.25) values. The light transmission of the film did not significantly decrease and nor did film transparency (p>0.05) with increased CLC. Incorporating CLC could not affect the film microstructure. The solubility of the gelatin based film incorporated with CLC was not affected, especially at a high level of addition (p>0.05). Inhibitory activity of the fish gelatin film against E.coli, S.aureus, L. innocua and S. cerevisiae was concentration dependent.
These findings suggested that CLC incorporation can improve mechanical, physico-chemical, and antimicrobial properties of the resulting films, thus allowing the films to become more applicable in active food packaging.
Microbial activity is a primary cause of deterioration in many foods and is often responsible for reduced quality and safety. Food-borne illnesses associated with E. coli O157:H7, S. aureus, S. enteritidis and L. monocytogenes are a major public health concern throughout the world . A number of methods have been employed to control or prevent the growth of these microorganisms in food, including the use of physical, biological, or chemical procedures. However, due to increasing concerns over the use of chemical agents as well as the high cost of physical treatments, there has been an increased demand for naturally-derived antimicrobial substances for food industries. Packaging incorporated with naturally active compounds is an alternative way to prolong shelf life of any processed foods. In general, packaging is needed for the protection of food from the outside environment and/or from post contamination by microorganisms . Recently, much attention has been paid to the development of antimicrobial active systems by means of incorporating antimicrobial substances into and on food or onto the coatings of food packaging, which help to improve food safety and shelf life . Antimicrobial packaging is one of the most promising active packaging systems for effectively killing, inhibiting, or retarding the growth of food spoilage and pathogenic microorganisms. By these actions, the shelf life of the product is prolonged, and its quality and safety are better preserved .
Naturally occurring antimicrobials have been widely incorporated in gelatin film, including chitosan [5, 6], lysozyme , essential oils [8, 9], and nisin . Green tea extract has also been used as an active ingredient for active film preparation because of the polyphenols in tea that are good for both their antioxidant and antimicrobial activities [11, 12]. The active compounds can inhibit microorganism growth and food spoilage and thereby extend shelf life of processed foods by either reducing the microbial growth rate or extending the lag-phase of the target microorganisms [12, 13]. However, addition of these compounds to the film based material may affect the principal properties of the host material. Many researchers have reported that various bioactive additives such as enzymes , antimicrobial [5, 6], and antioxidant agents [11, 12] may get entrapped in the porous solid matrices in the gelatin and also be immobilized by covalent binding to a solid support, which would result in a change to the resulting film properties [14, 15].
Among all materials that are used for biodegradable film preparation, proteins are considered to provide the most desirable mechanics, gas barrier, and transparency properties, as well as provide high nutritional value . Gelatin-based films are thin, flexible, and transparent biodegradable materials. There are many applications for this type of film in food processing, packaging, drug recovery, and more [4, 8, 9, 17, 18]. The physical and structural properties of gelatin film are mainly influenced by a variety of factors such as molecular weight distribution, amino acid composition, plasticizer, and additives used, which play an important role in the mechanical, optical, and barrier properties of the resulting films . The addition of some other active compounds can alter the physico-chemical properties of the film, such as increased flexibility and moisture sensitivity, as well as other functional properties. The purpose of this study was to determine the mechanical, physico-chemical properties and inhibitory effects of the fish gelatin films against selected food spoilage microorganisms when incorporated with catechin-lysozyme.
Results and discussion
Effect of CLC on mechanical and physical properties of film
Tensile strength and elongation at break
Mechanical properties of fish gelatin based films incorporated with a combination of catechin and lysozyme at different concentrations
Treatment (% CLC)
Tensile strength (MPa)
33.49 ± 2.63 c
27.82 ± 10.52 a
34.34 ± 4.80 c
19.90 ± 9.40 a
26.95 ± 4.26 b
19.21 ± 11.84 a
3.31 ± 0.45 a
143.17 ± 7.81 b
Thickness and water vapor permeability of fish gelatin based films incorporated with a combination of catechin and lysozyme at different concentrations
Treatment (% CLC)
WVP (n=5) (10−8 g·mm·h-1·cm-2·Pa-1)
0.036 ± 0.0012 a
12.60 ± 0.33 d
0.038 ± 0.0021 b
10.77 ± 0.71 c
0.037 ± 0.0022 ab
8.43 ± 0.53 b
0.039 ± 0.0022 b
6.50 ± 0.43 a
Effect of CLC on physico-chemical properties of film
Water vapor permeability
The WVP of the gelatin based film incorporated with CLC at various concentrations is presented in Table 2. The differences in WVP were observed among films prepared from different CLC concentrations (p<0.05). When increasing the CLC concentration incorporated into the film, decreased WVP was observed. The WVP of the resulting films decreased from 12.60 (control) to 6.50 (0.5% CLC), which shows the highest and lowest WVP of the films, respectively. The structural modifications of the gelatin network might be occurred when CLC was added. The lysozyme peptide or functional group of catechin will react chemically with a gelatin to form a stable bond, which results in the formation of a more complex structure of the resulting film. A high WVP of edible film is not desirable with respect to its usage and performance . From the results, the addition of CLC could improve the barrier properties of the film by lowering moisture transfer between the food and the surrounding atmosphere when this film was applied to any heterogeneous food product. The water vapour transfer process in films depends on the hydrophilic-hydrophobic ratio of the film constituents . Additionally, more pronounced protein cross-linking of the film along with more rigid and denser structures might retard water diffusion through the films. Film with higher thickness can absorb more water from the environment . These results are in contrast with the results of Bower et al. , who reported that when the concentration of lysozyme concentration increased from 0 to 0.1%, decreased of WVP was observed in antimicrobial gelatin film.
Color parameters of fish gelatin based films incorporated with a combination of catechin and lysozyme at different concentrations
Treatment (% CLC)
91.95 ± 0.08 c
−1.29 ± 0.01 a
2.25 ± 0.07 a
88.43 ± 0.43 a
−0.01 ± 0.06 d
3.88 ± 0.34 b
88.62 ± 0.26 ab
−0.15 ± 0.14 c
5.02 ± 0.23 c
88.98 ± 0.51 b
−0.41 ± 0.05 b
5.44 ± 0.11 d
Light transmission and transparency
Light transmission and transparency of fish gelatin based films incorporated with a combination of catechin and lysozyme at different concentrations
Treatment (% CLC)
3.34 ± 0.01 ab
3.36 ± 0.01 ab
3.34 ± 0.01 ab
3.33 ± 0.01 a
Surface morphology and film microstructure
The surface morphology of gelatin based film incorporated with CLC at various concentrations is shown in Figure 1. All film specimens were highly transparent, but some of them, although highly translucent, presented a slightly yellow to brown appearance. No significant differences between samples were observed in terms of transparency as indicated by the appearance of letters on the background. However, there was more turbidity of the gelatin film present in the white background when the concentration of CLC increased compared to the control, especially for the film containing 0.5% CLC. These results were similar to the light transmission and transparency of the gelatin film as mentioned above. Based on the film surface morphology property, applications of edible gelatin film incorporated with CLC at high levels may be limited to only some food products e.g. red meat products or sausages. According to the transparency of the film, customers can clearly see the product packed inside the package, making it is easier deciding whether or not to buy that product.
Film solubility and protein solubility
Film solubility and protein solubility of fish gelatin based films incorporated with a combination of catechin and lysozyme at different concentrations
Treatment (% CLC)
Film solubility (%)
Protein solubility (%)
57.51 ± 2.04 b
54.78 ± 13.49 b
56.96 ± 2.69 b
46.94 ± 1.22 b
54.12 ± 1.87 b
29.43 ± 0.87 a
43.96 ± 2.57 a
16.98 ±1.49 a
Effect of CLC on film antimicrobial properties
Green tea polyphenols, especially catechin, have been reported to show inhibitory effects in vitro against food spoilage and pathogenic microorganisms including L. monocytogenes, E. coli, Salmonella typhi murium, S. aureus, Shigella frexneri, and V. Cholera[28, 29]. Catechin has been employed as a food additive for the purpose of antimicrobials and antioxidants for preserving of a wide range of foods, especially meat and meat products. The antimicrobial activity of catechin or any other tea polyphenols is probably due to the inhibition of DNA and RNA synthesis of bacterial cells or may be because of the inhibition of bacteria cytoplasmic membrane function and/or interference with bacteria energy metabolism . In addition, its antimicrobial activity is related to the attack on the phospholipid present in cell membranes, which causes increased permeability and leakage of cytoplasm. It may also be related to their interaction with enzymes located on the cell wall .
The findings suggest that incorporating more CLC into the film could lead to the modified fish gelatin based films, giving them some useful properties (i.e. improving mechanical and barrier properties) and allowing them to be used in areas of high-stretching film applications. However, more work is needed before industrial application. In addition, mixing CLC with film forming solution can improve the performance of gelatin films in terms of antimicrobial properties against food spoilage microorganism.
Chemicals and microbials
Catechin hydrolysate (C1251), lysozyme from chicken egg white (62971), bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA.). Beta-mercaptoethanol (βME) and electrophoresis reagents were obtained from Bio-Rad Laboratories (Hercules, CA, USA). Glycerol, and other analytical grade reagents were obtained from Merck (Darmstadt, Germany). Mueller-hinton broth (275730) was purchased from Difco (Kansas, USA). Mueller-hinton agar (105437) was purchased from Merck (Darmstadt, Germany).
Escherichia coli, Staphylococcus aureus and Saccharomyces cerevisiae were obtained from the Biological Laboratory, Scientific and Technological Instruments Center, Mae Fah Luang University, Chiang Rai, Thailand. Listeria innocua was purchased from the Department of Medical Sciences, Ministry of Public Health, Bangkok, Thailand. Cultures were streak-plated once a week and a single colony was inoculated into the appropriate media and incubated overnight in the appropriate atmospheric conditions.
Extraction of fish skin gelatin
Gelatin was extracted from the washed giant catfish skin by the method described in Jongjareonrak et al. . To remove non-collagenous proteins and pigments, the washed skin was soaked in 0.2 mol/l of NaOH with a skin to solution ratio of 1:10 (w/v) at 4°C with continuous gentle stirring. The solution was changed 3 times every 30 min. The alkaline-treated skin was then washed with tap water until it was neutral or slightly basic (pH<7.5). To swell the collagenous material in the fish skin matrix, the alkaline-treated skin was soaked in 0.05 mol/l of acetic acid with a skin to solution ratio of 1:10 (w/v) for 3 h at room temperature (25°C) with continuous gentle stirring. Acid-treated skin was washed as previously described. The swollen fish skin was soaked in distilled water with a skin/water ratio of 1:10 (w/v) at 45°C for 12 h with continuous stirring to extract the gelatin. The mixture was then filtered using two layers of cheesecloth. The resultant filtrate was then freeze-dried. The dried matter that came from the freeze-dried process was ground and then referred to as “gelatin powder”.
Preparation of gelatin films
The gelatin powder was mixed with distilled water to obtain the film-forming solution (FFS) with a protein concentration of 3% (w/v). Glycerol was used as a plasticizer at a concentration of 25% of protein. The solution was incubated at 60°C for 30 min in a water bath with occasional stirring. After the FFS was cooled to room temperature, the CLC (catechin and lysozyme combination with ratio 1:1) of the FFS at the levels of 0, 0.125%, 0.25, and 0.5% (w/v) were added into the FFS. De-aerated film forming solution (4 g) was cast onto a rimmed silicone resin plate (50x50 mm) and then evaporated at room temperature for 24 h before dried with a ventilated oven environmental chamber (model H110K-30DM; Seiwa Riko Co., Tokyo, Japan) at 25±0.5°C and 50±5% relative humidity (RH) for another 24 h. The obtained dried films were manually peeled.
Analyses of gelatin film properties
The film thickness was measured with a hand-held micrometer (Bial Pipe Gauge, Peacock Co., Tokyo, Japan). Nine random locations around each of the ten film samples were used for thickness determination.
Prior to testing the mechanical properties, the films were conditioned for 48 h at 50±5% RH at 25°C. The tensile strength (TS) and elongation at break (EAB) were determined by using a Universal Testing Machine (Lloyd Instrument, Hampshire, UK). Ten samples (2x5 cm) with an initial grip length of 3 cm were used for testing. The cross-head speed was set at 30 mm/min with 100N load cell use.
Water vapor permeability
w is the weight gain of the cup (g); x is the film thickness (m); A is the area of exposed film (m2); t is the time of gain (s); and (P2-P1)-1 is the vapor pressure differential across the film (Pa). The WVP was expressed as g m-1 s-1 Pa-1. A total of five samples were determined for each film.
Colour, light transmission and film transparency
The colour of the film was determined with a Hunter lab colour meter (Colour QuestXE, Virginia, USA) and expressed as L* (lightness), a* (redness/greenness), and b* (yellowness/blueness).
T600 is the fractional transmittance at 600 nm, and x is the film thickness (mm).
Surface morphology and gelatin film microstructure
The surface morphology of the gelatin films was examined by using a Canon Powershot A95 digital camera at 5.0 megapixels and illuminant D65 (Canon Marketing (Thailand) Co.,Ltd., Bangkok, Thailand). The gelatin films’ microstructure was observed by using a scanning electron microscope (SEM) (LE01450VP, Cambridge, UK) at an acceleration voltage of 10 kV and magnification at 1,200x and 5,000x.
Film solubility and protein solubility
The film solubility was determined according to the method of Gennadios et al. . The conditioned film samples were weighed and placed in a 50 ml centrifuge tube containing 10 ml of distilled water. The mixture was shaken at a speed of 250 rpm using a shaker (Heidolth Inkubator 10000, Schwabach, Germany) for 24 h. The un-dissolved debris was then removed by centrifugation at 3,000g for 20 min. The pellet was dried at 105°C for 24 h and weighed. The weight of the solubilized dry matter was calculated by subtracting its difference from the initial weight of the dry matter. It was then expressed as a percentage of the total weight.
To determine the protein solubility, the protein concentration in the supernatant after centrifugation was determined by using the Biuret method . Protein solubility was expressed as the percentage of the protein content of the film dissolved in water to the total protein in the film, which was solubilized with 1 M of NaOH for 24 h.
The protein patterns of gelatin, film forming solution, and the gelatin films were all analyzed by SDS–PAGE according to the method of Laemmli . To solubilize the films, the samples were mixed with a solubilizing solution (1% SDS). The mixtures were heated at 85°C for 1 h in a water bath to dissolve the proteins. The solubilized samples were then mixed with a sample buffer (0.5 M Tris–HCl, pH 6.8 containing 4% (w/v) SDS, and 20% (v/v) glycerol) and 10% (v/v) βME at a ratio of 1:1 (v/v). The samples (16 μg protein) were loaded into the polyacrylamide gel made of 7.5% running gel and of 4% stacking gel and subjected to electrophoresis at a constant current of 15 mA per gel by using a Mini Protean II unit (Bio-Rad Laboratories, Inc., Richmond, CA, USA). After electrophoresis, the gel was stained with 0.05% (w/v) Coomassie blue R-250 in 15% (v/v) methanol and 5% (v/v) acetic acid and then destained with 30% (v/v) methanol and 10% (v/v) acetic acid.
Antimicrobial testing of gelatin films
The films were tested for their inhibition against the target microorganisms: L. innocua, S. aureus, E. coli and S. cerevisiae by using an agar disc diffusion method. Cell densities of 108 cfu mL−1 were calculated and prepared from the cultures at approximately 7.50×108 cfu mL−1 for L. innocua and E. coli; 4.85×108 cfu mL−1 for S. aureus; and 4.75×108 cfu mL−1 for S. cerevisiae. Each film sample was cut into a circle of 5 mm in diameter and sterilized with UV light for 30 min  prior to being placed on an agar plate surface seeded with the test cultures. The plates were incubated for 1–2 days at the appropriate temperature for each culture. A clear zone formed around the film disc in the medium, and it was recorded as an indicator of inhibition for the microbial species. The evaluation of the inhibitory activity was carried out in quadruplicate by observing the inhibition zones.
The data was subjected to analysis of variance (ANOVA). A means comparison was carried out by Duncan’s multiple range tests. The analysis was performed by using an SPSS package (SPSS 10.0 for window, SPSS Inc, Chicago, IL).
This research was supported by Mae Fah Luang University and the Thailand Research Fund under the TRF Senior Research Scholar program. We also thank Matthew Robert Ferguson, Mahidol University International College for kindly providing suggestions and corrections for the manuscript. Mr. Samart Sai-UT is also appreciated for his work as a research assistant on this project.
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