Open Access

Simple isatin derivatives as free radical scavengers: Synthesis, biological evaluation and structure-activity relationship

Chemistry Central Journal20115:37

https://doi.org/10.1186/1752-153X-5-37

Received: 19 April 2011

Accepted: 1 July 2011

Published: 1 July 2011

Abstract

To develop more potent small molecules with enhanced free radical scavenger properties, a series of N-substituted isatin derivatives was synthesized, and the cytoprotective effect on the apoptosis of PC12 cells induced by H2O2 was screened. All these compounds were found to be active, and N-ethyl isatin was found with the most potent activity of 69.7% protective effect on PC12 cells. Structure-activity relationship analyses showed the bioactivity of N-alkyl isatins decline as the increasing of the chain of the alkyl group, furthermore odd-even effect was found in the activity, which is interesting for further investigation.

Background

Oxidative stress has been implicated as a major role in the onset and progression of a vast variety of clinical abnormalities including neurodegenerative disorders. Free radicals play important roles in many physiological and pathological conditions [1]. In general, the generation and scavenging of oxygen free radicals is balanced and any imbalance or excessive amounts of active radicals may contribute to disease development. It has been found that free radical reactions can produce deleterious modifications in membranes, proteins, enzymes, and DNA [2], increasing the risk of diseases such as cancer [3], Alzheimer's [4], Parkinson's [5], angiocardiopathy [6], arthritis [7], asthma [8], diabetes [9], and degenerative eye disease [10]. Therefore, it is important to find effective scavengers of free radicals for prevention and treatment of such disorders.

Isatin is an endogenous indole present in mammalian tissues and fluids [11]. The substance was initially discovered as a component of endogenous monoamine oxidase (MAO) inhibitory activity, tribulin, and subsequently identified as a selective inhibitor of MAO B [12]. Further investigations have shown that isatin acts as an antagonist of both atrial natriuretic peptidestimulated and nitric oxide-stimulated guanylate cyclase activity [1315]. Isatin has a distinct and discontinuous distribution in rat brain and other tissues; the highest concentrations in the brain are found in the hippocampus and cerebellum [10]. Many Isatin derivatives, such as isatin hydrazono, isatin Mannich bases, isatin based spiroazetidinones and 3-(methylene)indolin-2-ones, have also been reported to possess neuroprotection activity [1619].

To develop more potent small molecules with enhanced free radical scavenger properties, a series of N-substituted isatin derivatives was synthesized by substitution reactions (as shown in Scheme 1), and the cytoprotective effect on the apoptosis of PC12 cells induced by H2O2 was screened.
Scheme 1

Synthesis of N -substituted isatin derivatives.

Results and Discussion

Chemistry

The N-substituted isatin derivatives were synthesized by reactions of substitution reaction. The reaction between isatin and halohydrocarbon has been reported being carried out in the presence of NaOEt using EtOH as solvent or in the presence of NaH using DMF as solvent [16]. The reactants and the solvents involved in the reactions must be anhydrous. To develop a simple method to synthesize N-substituted isatin derivatives, we firstly screened the effect of the base and solvent on the yield of the reaction of isatin and bromoethane (C2H5Br), and the results was shown in Table 1.
Table 1

The substitution reaction between isatin and bromoethane

No.

Solvent

Base

Time (h)

Yield (%)

1

DMF

Na2CO3

24

33

2

DMF

K2CO3

12

89

3

DMF

NaOH

12

40

4

DMF

KOH

12

41

5

DMF

TEA

24

60

6

MeOH

K2CO3

24

12

7

THF

K2CO3

24

25

8

DCM

K2CO3

24

19

9

Acetonitrile

K2CO3

24

15

In this reaction, the protons transfers from N-H (a Brösted acid) to a Brösted or Lewis base via the hydrogen-bonded covalent and ionic complexes [20], producing the isatin anion which is the nucleophilic reactant to the halohydrocarbon. Higher solvent polarity can promote the proton-transfer equilibrium and leads to the higher yield [20]. From this table, it can be found that K2CO3-DMF system was an effective promotion for this reaction and other base-solvent systems were not effective with the yield no more than 60%. The possible reason might be that weak base can not help the proton transfer at the beginning effectively, but the too strong bases will lead to the substitution reaction between bromoethane and OH-. DMF exhibits the highest yield of 89% with K2CO3 for its highest solvent polarity, so the K2CO3-DMF was selected as the reactant reaction system in the following synthesis, and the results were shown in Table 2.
Table 2

Synthesis of N-substituted isatin derivatives

Compound

R

Time (h)

Yield (%)

1

H

_

_

2

CH3

4

87

3

C2H5

12

89

4

(CH2)2CH3

12

89

5

(CH2)3CH3

24

93

6

(CH2)4CH3

24

90

7

(CH2)5CH3

24

95

8

CH2CH = CH2

12

93

9

CH2C6H5

12

90

10

CH2COOC2H5

12

93

11

C2H4Cl

24

79

12

C2H4Br

24

83

Bioactivity

The chemical modification of lead compound 1, focusing on the N-substituent, was carried out to further improve the free scavenging ability. A series of new N-substituted isatin derivatives (compounds 2-12) was synthesized. The free radical scavenging properties of these derivatives were evaluated to elucidate structure-activity relationships. The protective effect on the apoptosis of PC12 cells induced by H2O2 by free radical scavenging of these compounds against H2O2 were evaluated by cell survival assay in PC12 cells using a reported method [21]. The results were given in Table 3.
Table 3

Inhibitory and protective effects of N-substituted isatin derivatives

Compound

Inhibitory effect/% a

Protective effect/% b

 

200 μg/ml

20 μg/ml

2 μg/ml

200 μg/ml

20 μg/ml

2 μg/ml

1

92.0

0.0

2.6

-

20.1

40.4

2

60.6

2.7

5.0

-

31.4

50.9

3

45.6

0.4

7.0

-

38.2

69.8

4

43.0

3.3

0.0

-

10.1

39.7

5

53.3

0.0

0.0

-

30.0

60.8

6

51.9

0.0

0.0

-

8.2

24.1

7

50.4

0.0

0.0

0

15.5

20.9

8

54.0

2.1

0.0

-

14.8

69.5

9

63.1

0.0

0.0

-

9.3

51.3

10

67.9

0.0

5.3

-

46.6

54.5

11

63.5

20.5

5.6

-

0

25.1

12

61.6

24.1

12.3

-

0

62.1

VE

  

0.0

  

22.5

a) Inhibition of PC12 cell growth; b) protective effect on the apoptosis of PC12 cells induced by H2O2.

From the table, we can find almost all of the compounds showed potent activity at the condensation of 2 μg/ml, which were more effective than VE ((±) α-Tocophreol with the percentage of 22.5%). There is a noteworthy phenomenon that the activities of all compounds at the condensation of 2 μg/ml are more potent than that at the condensation of 20 μg/ml, and the mechanism will be interesting for the further investigation. Compound 3 and 8 exhibited the most potent activity with the protective effect of 69.8% and 69.5% at the condensation of 2 μg/ml respectively, which are more potent than that at the condensation of 20 μg/ml.

Almost all of these compounds were weakly cytotoxic to PC12 cells at the concentrations of 2-20 μg/ml except compound 11 and 12. Almost all compounds are cytotoxic to PC12 cells at the concentrations of 200 μg/ml, the PC12 cells inhibitory effects are more than 40%. Based on the factors, we can conclude the addition of halogenous atom in the substituents (compound 11 and 12) enhance the cytotoxicity at the concentrations of 2-20 μg/ml.

The substitution reaction between isatin and halohydrocarbon (C1 to C6) gave compounds 2-7, which provided the appropriate material for the structure-activity relationship analyses. The cytoprotective activities of N-substituted isatin derivatives with the alkyl group containing one to six carbon atoms were shown in Figure 1. The activity approximately declines as the increase of the chain of the alkyl group. With a further analysis, it was found that there was a clear odd-even effect in these activities. The activities of N-substituted isatin derivatives with odd carbon atoms alkyl group (one, three and five carbon atoms, corresponding compound 2, 4 and 6, marked with solid pillars in Figure 1) decline as the chain of the alkyl group increases, and the same regulation can be found in the activities of the N-substituted isatin derivatives with even carbon atoms alkyl group (two, four and six carbon atoms, corresponding compound 3, 5 and 7, marked with virtual pillars in Figure 1). This regulation exhibits both under the condensation of 2 μg/ml and 20 μg/ml, and the activities of N-substituted isatin derivatives with even carbon atoms alkyl group are more potent than the that of N-substituted isatin derivatives with parallel odd carbon atoms alkyl group. Besides, by the structure-activity relationship analyses, it was found that the unsaturated bond of the substituent (compound 8-10) can improve the activity compared with the other substituents with similar carbon atoms.
Figure 1

The cytoprotective activities of N -substituted isatin derivatives with the alkyl group containing 1-6 carbon atoms (The corresponding compounds are compounds 2-7.).

Experimental

All starting materials and solvents (A.R. grade) were commercially available and were used without further purification. NMR spectra were recorded using a Bruker Drx-400 spectrometer operating at 400 MHz for 1H. Mass spectra were recorded on a Micromass Platform spectrometer using a direct-inlet system operating in the electron impact (EI) mode at 75 eV. Elemental analyses were obtained using a Carlo Erba 1106 elemental analyzer.

General synthesis of N-alkyl substituted isatin derivatives

Isatin (1 mmol) and halohydrocarbon (1.2 mmol) were dissolved in DMF (20 ml), and 3 mmol anhydrous K2CO3 was added. The mixture was stirred under room temperature until the disappearance of isatin, as evidenced by thin-layer chromatography. The solvent was removed in vacuo and the residue was separated by column chromatography (silica gel, petroleum ether/ethyl acetate = 20:1), giving N-alkyl substituted isatin compound (compound 2-12).

1-Methylindoline-2,3-dione (Compound 2)

1H-NMR (D6-DMSO, 400 MHz): 7.66 (1 H, td, J = 1.2, 7.6 Hz), 7.52 (1 H, d, J = 7.6 Hz), 7.12 (2 H, t, J = 7.6 Hz), 3.12 (3 H, s); MS (EI) m/z: 161 (M+); Anal. Found: C, 67.01; H, 4.40; N, 8.66 (%). Calc. for (C9H7NO2): C, 67.07; H, 4.38; N, 8.69 (%).

1-Ethylindoline-2,3-dione (Compound 3)

1H-NMR (CDCl3, 400 MHz): 7.57 (2 H, m), 7.09 (1 H, t, J = 7.6 Hz), 6.89 (1 H, d, J = 7.6 Hz), 3.76 (2 H, q, J = 7.6 Hz), 1.29 (3 H, t, J = 7.6 Hz); MS (EI) m/z: 175 (M+); Anal. Found: C, 68.59; H, 5.22; N, 8.01 (%). Calc. for (C10H9NO2): C, 68.56; H, 5.18; N, 8.00 (%).

1-Propylindoline-2,3-dione (Compound 4)

1H-NMR (D6-DMSO, 400 MHz): 7.58 (1 H, d, J = 6.8 Hz), 7.55 (1 H, t, J = 7.6 Hz), 7.09 (1 H, t, J = 7.6 Hz), 6.88 (1 H, d, J = 8 Hz), 3.67 (2 H, t, J = 7.2 Hz), 1.72 (2 H, m, J = 7.2-7.6 Hz), 0.98 (3 H, t, J = 7.6 Hz); MS (EI) m/z: 189 (M+); Anal. Found: C, 69.88; H, 5.89; N, 7.35 (%). Calc. for (C11H11NO2): C, 69.83; H, 5.86; N, 7.40 (%).

1-Butylindoline-2,3-dione (Compound 5)

1H-NMR (CDCl3, 400 MHz) δ: 7.60 (2 H, m), 7.12 (1 H, t, J = 7.6 Hz), 6.91 (1 H, d, J = 8.4 Hz), 3.73 (2 H, t, J = 7.6 Hz), 1.69 (2 H, m), 1.42 (2 H, m), 0.98 (3 H, t, J = 7.2 Hz); MS (EI) m/z: 203 (M+); Anal. Found: C, 70.90; H, 6.59; N, 6.90 (%). Calc. for (C12H13NO2): C, 70.92; H, 6.54; N, 6.89 (%).

1-Pentylindoline-2,3-dione (Compound 6)

1H-NMR (CDCl3, 400 MHz) δ: 7.60 (2 H, m), 7.12 (1 H, t, J = 7.6 Hz), 6.91 (1 H, d, J = 8.0 Hz), 3.72 (2 H, t, J = 7.6 Hz), 1.71 (2 H, m), 1.37 (4 H, m), 0.91 (3 H, t, J = 6.8 Hz); MS (EI) m/z: 217 (M+); Anal. Found: C, 71.88; H, 7.00; N, 6.44 (%). Calc. for (C13H15NO2): C, 71.87; H, 6.96; N, 6.45 (%).

1-Hexylindoline-2,3-dione (Compound 7)

1H-NMR (CDCl3, 400 MHz) δ: 7.60 (2 H, m), 7.11 (1 H, t, J = 7.6 Hz), 6.90 (1 H, d, J = 7.6 Hz), 3.72 (2 H, t, J = 7.6 Hz), 1.70 (2 H, m), 1.31-1.38 (6 H, m), 0.89 (3 H, t, J = 6.4 Hz); MS (EI) m/z: 231 (M+); Anal. Found: C, 72.72; H, 7.40; N, 6.01 (%). Calc. for (C14H17NO2): C, 72.70; H, 7.41; N, 6.06 (%).

1-Allylindoline-2,3-dione (Compound 8)

1H-NMR (D6-DMSO, 400 MHz): 7. 63 (1 H, t, J = 7.6 Hz), 7.55 (1 H, d, J = 7.2 Hz), 7.12 (1 H, t, J = 7.6 Hz), 7.04 (1 H, d, J = 7.6 Hz), 5.84 (1 H, m, J = 5.2-5.6 Hz), 5.32 (1 H, d, J = 17.2 Hz), 5.18 (1 H, d, J = 10.4 Hz), 4.30 (2 H, d, J = 4.8 Hz); MS (EI) m/z: 187 (M+); Anal. Found: C, 70.60; H, 4.84; N, 7.49 (%). Calc. for (C11H9NO2): C, 70.58; H, 4.85; N, 7.48 (%).

1-Benzylindoline-2,3-dione (Compound 9)

1H-NMR (D6-DMSO, 400 MHz) δ: 7.56 (2 H, m), 7.42 (2 H, d, J = 7.6 Hz), 7.30 (2 H, t, J = 7.6 Hz), 7.27 (1 H, m), 7.10 (1 H, t, J = 7.6 Hz), 6.96 (1 H, m), 4.90 (2 H, s); MS (EI) m/z: 233 (M+); Anal. Found: C, 75.99; H, 4.65; N, 5.92 (%). Calc. for (C15H11NO2): C, 75.94; H, 4.67; N, 5.90 (%).

Ethyl 2-(2,3-dioxoindolin-1-yl)acetate (Compound 10)

1H-NMR (CDCl3, 400 MHz) δ: 7.62 (1 H, d, J = 7.6 Hz), 7.57 (1 H, t, J = 7.6 Hz), 7.14 (1 H, t, J = 7.6 Hz), 6.77 (1 H, d, J = 7.6 Hz), 4.47 (2 H, s), 4.22 (2 H, q, J = 7.2 Hz), 1.26 (3 H, t, J = 7.2 Hz); MS (EI) m/z: 233 (M+); Anal. Found: C, 61.84; H, 4.72; N, 6.00 (%). Calc. for (C12H11NO4): C, 61.80; H, 4.75; N, 6.01 (%).

1-(2-Chloroethyl)indoline-2,3-dione (Compound 11)

1H-NMR (D6-DMSO, 400 MHz) δ: 7.67 (1 H, td, J = 8, 1.2 Hz), 7.56 (1 H, dd, J = 7.6, 1.2 Hz), 7.29 (1 H, d, J = 8.0 Hz), 7.14 (1 H, dd, J = 7.6, 0.8 Hz), 4.10 (2 H, t, J = 6.4 Hz), 3.70 (2 H, t, J = 6.4 Hz); MS (EI) m/z: 211 (M+); Anal. Found: C, 58.86; H, 3.99; N, 13.70 (%). Calc. for (C10H8ClNO2): C, 58.82; H, 3.95; N, 13.72 (%).

1-(2-Bromoethyl)indoline-2,3-dione (Compound 12)

1H-NMR (D6-DMSO, 400 MHz) δ: 7.67 (1 H, td, J = 8, 1.2 Hz), 7.57 (1 H, dd, J = 7.6, 1.2 Hz), 7.29 (1 H, d, J = 8.0 Hz), 7.14 (1 H, dd, J = 7.6, 0.8 Hz), 4.11 (2 H, t, J = 6.4 Hz), 3.71 (2 H, t, J = 6.4 Hz); MS (EI) m/z: 254 (M+); Anal. Found: C, 47.31; H, 3.19; N, 5.50 (%). Calc. for (C10H8BrNO2): C, 47.27; H, 3.17; N, 5.51 (%).

Declarations

Acknowledgements

This work was financially supported by the grant from Scientific Research Program Funded by Shaanxi Provincial Education Department (Program No.11JK0560).

Authors’ Affiliations

(1)
College of Chemistry and Chemical Engineering, Xi'an Shiyou University
(2)
Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences

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