S.No. | Mobile phase composition | Proportions (v/v) | Observations |
---|---|---|---|
1 | Acetone: EDTA: methanol: 1% ammonia solution | 65:5:10:10 | Poor resolution of peaks |
2 | Acetone: EDTA: methanol: 1% ammonia solution | 65:5:10:15 | Resolution problem between peaks |
3 | Chloroform: acetonitrile: methanol: 1% ammonia solution | 11:49:25:15 | Poor resolution between peaks |
4 | Chloroform: dimethylformamide: acetone: 1% ammonia solution | 20:20:40:30 | Longer development time, high noise |
5 | Ethyl acetate, acetone, methanol, and 1% ammonia solutiona,b | 4.4:19.6:10:6 | Sharp peak with good resolution at Rf 0.27Â \(\pm\)Â 0.02 |
6 | Ethyl acetate: acetonitrile: methanol: 1% ammonia solution | 4.4:19.6:10:6 | impurity peak overlap on analyte peak |
7 | Ethyl acetate: dimethylformamide: acetone: 1% ammonia solution | 10:22:50:18 | Peak resolution problem |
8 | Water: methanol: acetone: dichloromethane | 10:70:20:60 | Longer development time |