Table 1 Factors used in central composite design and their selected levels
Factor | Unit | Low | High | -alpha | +alpha |
|---|---|---|---|---|---|
% of organic phase | % | 40.0 | 60.0 | 40.0 | 60.0 |
pH of the buffer | 3.0 | 7.0 | 3.0 | 7.0 | |
Flow rate | mL/min | 0.2 | 0.4 | 0.2 | 0.4 |
Factor | Unit | Low | High | -alpha | +alpha |
|---|---|---|---|---|---|
% of organic phase | % | 40.0 | 60.0 | 40.0 | 60.0 |
pH of the buffer | 3.0 | 7.0 | 3.0 | 7.0 | |
Flow rate | mL/min | 0.2 | 0.4 | 0.2 | 0.4 |