Table 1 Analytical performance data for ASP and ROX determination using the developed spectrophotometric methods

λ (nm)

242.5 & 255.5

234 & 280

238.5

252 & 286

254 & 297

293

Range (µg/mL)

4-40 µg/mL

2–20 µg/mL

Linearity

 Slope

0.0054

0.0221

− 0.0306

0.0291

0.1444

0.5056

 Intercept

0.001

− 0.0099

0.0277

0.0003

0.1495

0.1667

 Correlation coefficient

0.9997

0.9997

0.9999

0.9995

0.9998

0.9998

Accuracy^a

 Mean ± RSD

100.26 ± 1.057

100.72 ± 0.957

99.30 ± 0.799

99.91 ± 0.621

101.38 ± 0.490

99.57 ± 0.614

Precision (% RSD)

 Repeatability^b

1.132

1.078

1.213

1.037

1.096

1.158

 Intermediate precision ^c

1.288

1.319

1.499

1.395

1.214

1.361

Limits

 Limit of detection, LOD

0.61

0.66

0.53

0.45

0.29

0.26

 Limit of quantitation, LOQ

1.85

2

1.63

1.37

0.92

0.79