Determination of antihistaminic drugs alcaftadine and olopatadine hydrochloride via ion-pairing with eosin Y as a spectrofluorimetric and spectrophotometric probe: application to dosage forms

Table 2 Accuracy of the proposed spectroscopic methods for the determination of ALC and OLO using the standard addition method

Amount taken	Amount added	Amount found	% Recovery ± SD^a
Method A
200 ng mL⁻¹	0	198.83 ng mL⁻¹	99.42 ± 1.84
200 ng mL⁻¹	300 ng mL⁻¹	502.24 ng mL⁻¹	100.44 ± 1.69
200 ng mL⁻¹	800 ng mL⁻¹	1014.33 ng mL⁻¹	101.43 ± 1.20
200 ng mL⁻¹	1300 ng mL⁻¹	1502.35 ng mL⁻¹	100.16 ± 1.24
Method B
1.0 μg mL⁻¹	0	1.001 μg mL⁻¹	100.11 ± 1.09
1.0 μg mL⁻¹	1.0 μg mL⁻¹	2.003 μg mL⁻¹	100.02 ± 0.84
1.0 μg mL⁻¹	3.0 μg mL⁻¹	4.021 μg mL⁻¹	100.52 ± 0.72
1.0 μg mL⁻¹	5.0 μg mL⁻¹	5.994 μg mL⁻¹	99.90 ± 1.47
Method C
200 ng mL⁻¹	0	200.50 ng mL⁻¹	100.25 ± 1.30
200 ng mL⁻¹	200 ng mL⁻¹	398.24 ng mL⁻¹	99.56 ± 0.65
200 ng mL⁻¹	600 ng mL⁻¹	803.25 ng mL⁻¹	100.41 ± 0.68
200 ng mL⁻¹	1200 ng mL⁻¹	1192.66 ng mL⁻¹	99.39 ± 0.33
Method D
2.0 μg mL⁻¹	0	2.002 μg mL⁻¹	100.11 ± 1.55
2.0 μg mL⁻¹	2.0 μg mL⁻¹	3.992 μg mL⁻¹	99.79 ± 0.78
2.0 μg mL⁻¹	4.0 μg mL⁻¹	5.995 μg mL⁻¹	99.91 ± 1.28
2.0 μg mL⁻¹	6.0 μg mL⁻¹	8.059 μg mL⁻¹	100.73 ± 0.25

ISSN: 2661-801X