Determination of antihistaminic drugs alcaftadine and olopatadine hydrochloride via ion-pairing with eosin Y as a spectrofluorimetric and spectrophotometric probe: application to dosage forms

Table 1 The regression and validation parameters for the proposed spectroscopic methods for the determination of ALC and OLO

Parameter	Method A	Method B	Method C	Method D
Linear range (µg mL⁻¹)	0.15–2.0	0.8–8.0	0.20–2.0	1.0–10
Slope	0.415	0.091	0.384	0.049
SD of slope (S_b)	5.10	0.0013	0.005	0.0005
Intercept	0.76	0.0086	0.94	− 0.001
SD of intercept (S_a)	5.75	0.0059	5.301	0.003
Correlation Coefficient	0.9997	0.9996	0.9996	0.9997
SD of residuals (S_{y, x})	8.20	0.00031	7.56	0.004
LOD	46.67 ng mL⁻¹	0.21 µg mL⁻¹	45.52 ng mL⁻¹	0.21 µg mL⁻¹
LOQ	138.40 ng mL⁻¹	0.65 µg mL⁻¹	137.93 ng mL⁻¹	0.63 µg mL⁻¹

ISSN: 2661-801X