Proposed method | Reported spectrophotometric method [36] | Reported HPLC method [37] | |
---|---|---|---|
GAPI | |||
NEMI | |||
AGREE | |||
Eco-Scale | Item Penalty points 1-Reagent Water 0 2-Spectrofluorimeter 0 3-Occupational hazard 0 4-Waste 3 Total penalty points 3 Eco- Scale score 97 | Item Penalty points 1-Reagent NaOH 1 2-UV- Vis Spectrometer 0 3-Occupational hazard 0 4-Waste 3 Total penalty points 4 Eco- Scale score 96 | Item Penalty points 1-Reagent Methanol 12 Acetonitrille 8 Phosphate buffer 0 2-HPLC–UV 1 3-Occupational hazard 0 4-Waste 3 Total penalty points 24 Eco- Scale score 76 |