Table 1 Analytical performance of the suggested technique and other reported methods
Reference | Range (µg ml−1) | LOD (µg ml−1) | Technique | Assessment of greenness | Application | ||
|---|---|---|---|---|---|---|---|
PLN | CFX | PLN | CFX | ||||
The proposed method | 0.02–0.5 | 0.5–5.0 | 0.004 | 0.103 | The assay principle based on first-order synchronous spectrofluorometric scan using Δ λ = 40 nm in which PLN peak amplitudes were recorded at 283.4 nm, meanwhile CFX was measured at 326.2 nm in the same scans | The method greenness was assessed and confirmed by four assessment tools; GAPI, NEMI, AGREE and Analytical Eco Scale Score | PLN and CFX in their pure forms, single and combined dosage forms |
[36] | 5.0–30.0 | 1.0‐12.0 | 1.16 | 0.110 | Dual wavelength spectrophotometric simultaneous determination of PLN and CFX. The wavelengths selected for assay of CFX were 272.09 nm and 305.85 nm, and for PLN were 256.80 nm and 282.96 nm using 0.1 M NaOH as solvent | No assessment | PLN and CFX in pure forms and combined dosage forms only |
[37] | 5.0–30.0 | 150–900 | 0.220 | 2.19 | A stability-indicating reversed-phase HPLC method was developed for simultaneous assay of PLN and CFX using C18 column and a mobile phase consisting of Water: Acetonitrile: Triethylamine (85: 15: 0.1, v/v/v), pH 3 | No assessment | PLN and CFX in pure forms and combined dosage forms.only |