Table 6 The validation outcome and parameters of the established tools for CI and OR quantification

Linearity range μg/ml

2–15 μg/ml

3–25 μg/ml

2–15 μg/ml

3–25 μg/ml

Wavelength nm

280.1 nm

314.2 nm

272.0 nm

306.2 nm

Linear equation

Y = 0.2732 X + 0.0031

Y = 0.0778 X + 0.002

Y = 0.2091 X + 0.0026

Y = 0.0571 X + 0.0042

R2

0.9999

0.9998

1

0.9999

Mean% ± SD^a

100.63 ± 1.23

100.50 ± 1.02

100.20 ± 1.35

100.11 ± 0.83

Repeatability^b

0.94

0.91

1.10

1.41

Intermediate Precision^b

1.10

1.75

1.02

1.45

DL μg/ml

0.107

0.163

0.139

0.209

QL μg/ml

0.324

0.495

0.422

0.634