AQbD TLC-densitometric method approach along with green fingerprint and whiteness assessment for quantifying two combined antihypertensive agents and their impurities

Table 6 Green and whiteness assessment with comparison between the proposed and reported methods

NO	Analytical method	NEMI	GAPI	Eco-scale	Whiteness %
1	Developed TLC-densitometric method	〹	〹	85	105.30
2	Reported method* [18]			82	101.50
3	Reported method** [10]			77	88.20
4	Reported method*** [17]			71	80.20

*Reported method [18]: is capillary electrophoresis for determination of CPL and HCZ in presence of CDS, CTZ, SMD and HCZ impurity C in 3 min. New capillaries were flushed with 1 M NaOH for 5 min, followed by 0.1 M NaOH and water for 5 min each. Before every run, the capillaries were conditioned by flushing with methanol for 2 min, 0.1 M NaOH for 2 min, water for1min and BGE for 3 min. The selected working conditions: BGE, 100 mM borate buffer pH 8.55 (8.48–8.62), 64 mM (60–68 mM) sodium cholate, 6.1% v/v (5.4–6.8% v/v) n−butanol, 12 mM (11–13 mM) γ−CD. Voltage, 27 kV (26–28 kV), temperature, 21 °C and measured current was about 85 μA. Separations were carried out in a fused−silica capillary (50 mm inner diameter, 375 mm outer diameter, total length 33.0 cm) with a detection window at 24.5 cm. Solvent consumption calculated by capillary electrophoresis calculator program
**Reported method [10]: is HPLC for determination of CPL and HCZ using methanol: water (45:55, v/v), as the mobile phase pumped at flow rate 1 mL/min for 5 min. with UV detection at 210 nm
***Reported method [17]: is HPLC for determination of CPL and HCZ in presence of CDS and SMD using methanol: 0.05% aqueous phosphoric acid (25: 75, v/v) pumped at 2 mL/min for 8 min. the flow rate was increased to 4.5 mL/min for the next 7 min. and the methanol was increased to 45%, then methanol ratio was decreased to 25% at flow rate 2 mL/min. for the last 5 min

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