AQbD TLC-densitometric method approach along with green fingerprint and whiteness assessment for quantifying two combined antihypertensive agents and their impurities

Table 2 The calculated coefficients of the prediction models and P-values obtained from the ANOVA test results

Term	R_f CDS		R_s CTZ & HCZ		R_s HCZ & SMD
Term	Coefficient	p-value^a	Coefficient	p-value^a	Coefficient	p-value^a
I (Intercept)	0.7810	< 0.0001*	1.0807	< 0.0001*	1.0881	< 0.0001*
X (ethyl acetate ratio)	− 0.1943	< 0.0001*	− 0.1306	< 0.0001*	− 0.1609	0.0001*
Y (glacial acetic acid ratio)	− 0.0043	0.7690	− 0.0415	0.0011*	0.0041	0.7996
Z (plate length)	0.0515	0.0128*	− 0.0069	0.3116	0.0836	0.0025*
XY	− 0.0133	0.4196	− 0.0821	< 0.0001*	− 0.0548	0.0214*
XZ	− 0.0429	0.0373*	− 0.0216	0.0255*	− 0.0635	0.0127*
YZ	0.0272	0.1337	− 0.0511	0.0007*	0.0254	0.1903
XYZ	− 0.00004	0.9983	0.0258	0.0150*	0.0406	0.0661
X²	0.0175	0.6105	0.0274	0.1185	− 0.0271	0.4792
Y²	− 0.0724	0.0748	− 0.1273	0.0003*	− 0.0027	0.9430
Z²	− 0.0426	0.2375	0.0093	0.5473	− 0.0097	0.7933

*Values indicate significant effect on the corresponding response
Coefficient (slope); I, intercept; X, ethyl acetate ratio; Y, glacial acetic acid ratio; Z, plate length; R_f, retardation factor; R_s, resolution
^aSignificantly different at p ≤ 0.05

ISSN: 2661-801X