Table 1 Program of changing mobile phase flow rate for the developed HPLC method
Time (minutes) | Flow rate | MeOH ( containing 0.3% Triethylamine) | % 0.05M disodium hydrogen phosphate solution |
|---|---|---|---|
0–4.5 | 2 | 60 | 40 |
4.5–5.5 | 1.5 | 60 | 40 |
5.5–5.6 | 2 | 60 | 40 |
5.6–10 | 2 | 60 | 40 |