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Table 5 Robustness study for the developed HPLC method for the determination Lysine upon change in pH value and flow rate of the mobile phase

From: Development of chromatographic methods to determine multivitamins formulation depending on their solubility and polarity: comparative study using three greenness assessment tools

Parameters

Change in flow rate

 

Flow 0.9 mL/min

Flow 1 mL/min

Flow 1.1 mL/min

Lysine hydrochloride

K

Tailing factor

Theoretical plates

K

Tailing factor

Theoretical plates

K

Tailing factor

Theoretical plates

3.1

1.67

6834

2.85

1.55

6656

2.6

1.55

6373

Change in pH

pH 5.8

pH 6

pH 6.2

K

Tailing factor

Theoretical plates

K

Tailing factor

Theoretical plates

K

Tailing factor

Theoretical plates

2.71

1.42

8106

2.85

1.55

6656

2.85

1.52

6493

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BMC Chemistry

ISSN: 2661-801X

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