Table 4 Robustness study for the developed HPLC method for the determination of Ipriflavone, Ascorbic acid, Pyridoxine and Vitamin D3 upon change in column brand
Parameter | Change in column brand | |||||||
|---|---|---|---|---|---|---|---|---|
Column brand | Agilent Eclipse XDB-C18 | Thermo hypersil BDS C18 | ||||||
System suitability parameter | K | Tailing factor | Theoretical plates | Resolution* | K | Tailing factor | Theoretical plates | Resolution* |
Ascorbic acid | 2.1 | 1.09 | 13660 | 14.19 | 2.05 | 1.19 | 9651 | 15.8 |
Pyridoxine hydrochloride | 4.7 | 1.47 | 7810 | – | 5.07 | 1.02 | 8790 | – |
Ipriflavone | 9.5 | 0.99 | 81944 | 13.73 | 8.39 | 1.84 | 86875 | 12.57 |
Vitamin D3 | 12.1 | 1.05 | 47510 | – | 10.5 | 1.64 | 49834 | – |