Table 3 Robustness study for the developed HPLC method for the determination of Ipriflavone, Ascorbic acid, Pyridoxine and Vitamin D3 upon change in pH
Parameter | Change in pH | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
pH 4 | pH 4.2 | pH 4.4 | ||||||||||
System suitability parameter | K | Tailing factor | Theoretical plates | Rs* | K | Tailing factor | Theoretical plates | Rs* | K | Tailing factor | Theoretical plates | Rs* |
Ascorbic acid | 2.2 | 1.1 | 12698 | 14.22 | 2.11 | 1.09 | 13660 | 14.19 | 2.15 | 1.14 | 14505 | 14.15 |
Pyridoxine hydrochloride | 4.63 | 1.5 | 7946 | – | 4.73 | 1.47 | 7810 | – | 4.7 | 1.49 | 8210 | – |
Ipriflavone | 9.5 | 1.02 | 85490 | 13.68 | 9.52 | 0.99 | 81944 | 13.73 | 9.48 | 0.99 | 77579 | 13.78 |
Vitamin D3 | 11.9 | 1.1 | 47407 | – | 12.11 | 1.05 | 47510 | – | 12.2 | 1.06 | 48110 | – |