Table 2 Validation parameters of the proposed HPLC methods for the determination of Ipriflavone, Ascorbic acid, Pyridoxine, Vitamin D3 and Lysine

Wavelength (nm)

254 nm

254 nm

254 nm

254 nm

210 nm

Linearity range

2.5–80 µg/mL

7.5–90 µg/mL

10–100 µg/mL

1–60 µg/mL

20–200 µg/mL

Time of analysis (min/run)

14 min

6 min

Regression equation (Y = bX ‏ + a)*

Y = 51.229 X-6.3213

Y = 7.8682X + 14.192

Y = 118.9 X + 84.916

Y = 45.711 X + 1.5154

Y = 0.5756 X + 0.2469

Intercept (a)

6.3213

14.192

84.916

1.5154

0.2469

Slope (b)

51.229

7.8682

118.9

45.711

0.5756

Correlation coefficient (r)

0.9999

0.9997

1

0.9998

0.9998

Accuracy (mean ± %RSD)

100.53% ± 1.33

100.27% ± 1.44

99.76% ± 1.28

100.8% \(\pm\) 0.72

99.95% ± 1.41

Precision (% RSD)

 Repeatability^a

1.33

1.44

1.28

0.72

1.41

 Intermediate precision^b

1.22

1.27

1.3

1.07

1.16

Average recovery of the content of drug substances in Ultrcacalce^®capsules

 (Mean ± RSD)

32.86% ± 1.58^c

93.45% ± 1.16

95.31% ± 0.87

107.67% ± 1.57

106.88% ± 1.66