BMC Chemistry

Table 7 Assay results for determination of RUP, DES, FEX and MKT in synthetic mixtures by the proposed method

From: Environmentally benign liquid chromatographic method for concurrent estimation of four antihistaminic drugs applying factorial design approach

Analyte	Proposed method						Comparison methods [28, 31, 36]
Analyte	Concn. Taken (μg/mL)		Concn. Found (μg/mL)		% Found^a		% Found^a
Synthetic mixture of RUP/MKT (1:1)	RUP	MKT	RUP	MKT	RUP	MKT	RUP	MKT
	1.00	1.00	1.001	1.001	100.10	100.10	99.12	99.68
	8.00	8.00	7.901	8.008	98.76	100.10	101.13	100.54
	10.00	10.00	10.009	9.970	100.09	99.70	99.58	99.77
Mean ± SD					99.65 ± 0.77	99.97 ± 0.23	99.94 ± 1.05	100.00 ± 0.47
% Error					0.45	0.13	0.61	0.27
t					0.39 (2.78)^b	0.098 (2.78)^b
F					1.87 (19)^b	4.19 (19)^b
Synthetic mixture of DES/MKT (1:2)	DES	MKT	DES	MKT	DES	MKT	DES	MKT
	1.00	2.00	0.988	1.982	98.80	99.10	99.44	99.68
	2.00	4.00	2.014	4.027	100.70	100.68	100.87	100.54
	4.00	8.00	3.996	7.991	99.90	99.89	99.65	99.77
Mean ± SD					99.80 ± 0.95	99.89 ± 0.79	99.99 ± 0.77	100.00 ± 0.47
% Error					0.55	0.46	0.45	0.27
t					0.26 (2.78)^b	0.20 (2.78)^b
F					1.53 (19)^b	2.79 (19)^b
Synthetic mixture of MKT/FEX (1:12)	MKT	FEX	MKT	FEX	MKT	FEX	MKT	FEX
	1.00	12.00	0.991	11.825	99.10	98.54	99.68	101.41
	1.50	18.00	1.519	18.14	101.27	100.78	100.54	98.72
	2.00	24.00	1.99	23.946	99.50	99.78	99.77	100.42
Mean ± SD					99.96 ± 1.15	99.70 ± 1.12	100.00 ± 0.47	100.18 ± 1.36
% Error					0.67	0.65	0.27	0.78
t					0.06 (2.78)^b	0.47 (2.78)^b
F					5.97 (19)^b	1.47 (19)^b

^aEach result was the average of three separate determinations
^bThe tabulated t and F values at P = 0.05[46]

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