Table 1 A 2³ full factorial experimental design for RP-HPLC method with UV-detector for separation of DES, RUP, FEX and MKT

2³ full factorial design for FEX

2³ full factorial design for MKT

3

1

1

1

3.50

0.04

45

16.20

2.16

1.46

8

1

1

1

4.50

0.04

50

14.3

3.45

1.21

6

2

1

1

4.50

0.03

50

11.50

2.59

1.10

4

2

1

1

4.50

0.04

45

29.00

3.21

1.40

5

3

1

1

3.50

0.03

50

12.30

2.23

1.40

7

3

1

1

3.50

0.04

50

17.00

1.90

1.23

2

4

1

1

4.50

0.03

45

16.00

1.50

1.33

5

4

1

1

3.50

0.03

50

15.40

2.75

1.35

7

5

1

1

3.50

0.04

50

11.60

2.30

1.82

2

5

1

1

4.50

0.03

45

31.00

3.19

1.41

8

6

1

1

4.50

0.04

50

11.30

3.07

0.93

6

6

1

1

4.50

0.03

50

14.90

2.90

1.62

4

7

1

1

4.50

0.04

45

19.50

1.62

1.64

1

7

1

1

3.50

0.03

45

29.00

2.70

1.42

1

8

1

1

3.50

0.03

45

21.00

2.40

1.53

3

8

1

1

3.50

0.03

45

28.00

2.80

1.30

2³ full factorial design for DES

2³ full factorial design for RUP

4

1

1

1

4.50

0.04

45

11.00

2.60

1.52

5

1

1

1

3.50

0.03

50

11.14

2.37

1.16

1

2

1

1

3.50

0.03

45

10.54

0.80

1.84

2

2

1

1

4.50

0.03

45

13.00

2.91

1.44

3

3

1

1

3.50

0.04

45

10.43

0.82

1.83

4

3

1

1

4.50

0.04

45

12.30

3.33

1.29

8

4

1

1

4.50

0.04

50

8.00

3.75

1.42

3

4

1

1

3.50

0.04

45

13.10

2.81

1.44

5

5

1

1

3.50

0.03

50

8.60

1.75

1.60

8

5

1

1

4.50

0.04

50

9.72

3.21

1.11

2

6

1

1

4.50

0.03

45

10.0

1.96

1.68

7

6

1

1

3.50

0.04

50

10.50

2.81

1.10

6

7

1

1

4.50

0.03

50

8.20

2.91

1.45

1

7

1

1

3.50

0.03

45

13.000

2.51

1.54

7

8

1

1

3.50

0.04

50

8.50

2.60

1.40

6

8

1

1

4.50

0.03

50

9.64

2.78

1.26