Table 4 Assay validation parameters of the LC–MS/MS method
Analyte name | Linear equation | r | Linear Range (μg/mL) | LOD (μg/mL) | LOQ (μg/mL) | Stability (RSD%) | Recovery (RSD%) | Intra-day Precision (RSD%) | Inter-day Precision (RSD%) |
|---|---|---|---|---|---|---|---|---|---|
Morphine | Y = 15,123.4x−19903.9 | 0.9998 | 30.0–3000.0 | 0.030 | 0.100 | 0.6 | 100.7 | 3.3 | 4.1 |
Hydromorphone | Y = 146816x−24483.5 | 0.9991 | 3.6–360.0 | 0.040 | 0.110 | 1.1 | 97.4 | 2.7 | 3.5 |
Oxycodone | Y = 14,299.6x + 97,172.7 | 0.9987 | 4.25–425.0 | 0.040 | 0.130 | 1.2 | 95.4 | 1.4 | 2.5 |
Ketamine | Y = 16,904.4x + 219,548 | 0.9994 | 6.0–600.0 | 0.025 | 0.060 | 1.4 | 93.5 | 1.2 | 2.1 |
Tramadol | Y = 1039.3x + 7008.3 | 0.9995 | 3.0–300.0 | 0.060 | 0.200 | 0.4 | 100.1 | 1.0 | 1.2 |
Dezocine | Y = 3903.3x + 168,952 | 0.9992 | 30.0–3000.0 | 0.004 | 0.015 | 0.5 | 101.7 | 0.9 | 1.8 |
Ropivacaine | Y = 7110.7x + 7625.8 | 0.9996 | 1.0–100.0 | 0.002 | 0.005 | 1.8 | 98.3 | 1.3 | 2.5 |
Remifentanil | Y = 71,600.1x + 200,119 | 0.9998 | 1.5–150.0 | 0.002 | 0.005 | 1.3 | 95.4 | 1.6 | 3.9 |
Butorphanol | Y = 8804.7x + 134,721 | 0.9991 | 5.0–500 | 0.005 | 0.020 | 0.6 | 101.5 | 1.3 | 2.1 |
Bupivacaine | Y = 259878x + 93,774.5 | 0.9993 | 0.3–30.0 | 0.006 | 0.020 | 1.1 | 94.9 | 0.6 | 1.5 |
Droperidol | Y = 6196.8x + 34,185.8 | 0.9990 | 2.5–250.0 | 0.008 | 0.025 | 0.3 | 102.6 | 1.7 | 1.6 |
Fentanyl | Y = 61,017.9x + 90,943.7 | 0.9995 | 1.6–160.0 | 0.003 | 0.010 | 0.6 | 97.5 | 0.9 | 1.9 |
Lornoxicam | Y = 512.98x + 7110.4 | 0.9992 | 48.0–4800.0 | 0.048 | 0.192 | 1.0 | 98.8 | 1.4 | 2.7 |
Sufentanil | Y = 178346x + 133,993 | 0.9990 | 1.0–100.0 | 0.002 | 0.008 | 1.4 | 99.6 | 2.1 | 3.4 |