Table 3 Assay validation parameters of the HPLC–DAD method
Analyte name | Linear equation | r | Linear Range (μg/mL) | LOD (μg/mL) | LOQ (μg/mL) | Stability (RSD%) | Recovery (RSD%) | Intra-day Precision (RSD%) | Inter-day Precision (RSD%) |
|---|---|---|---|---|---|---|---|---|---|
Morphine | Y = 58.897X–2.3304 | 0.9999 | 20.0–200.0 | 0.15 | 0.50 | 0.9 | 99.6 | 0.9 | 1.5 |
Hydromorphone | Y = 7.7426X−2.1182 | 0.9996 | 20.0–200.0 | 0.20 | 0.60 | 1.4 | 99.4 | 0.4 | 1.1 |
Oxycodone | Y = 24.252X−3.9545 | 0.9998 | 20.0–200.0 | 0.30 | 1.00 | 0.8 | 98.2 | 0.9 | 1.6 |
Ketamine | Y = 6.4043X−2.0872 | 0.9991 | 20.0–200.0 | 2.50 | 8.00 | 1.7 | 100.2 | 0.6 | 1.1 |
Tramadol | Y = 9.2999X−10.616 | 0.9996 | 20.0–400.0 | 0.60 | 1.80 | 0.2 | 100.3 | 0.5 | 1.6 |
Dezocine | Y = 107.13X−23.168 | 0.9990 | 30.0–150.0 | 1.80 | 5.00 | 0.9 | 101.9 | 0.7 | 1.9 |
Ropivacaine | Y = 15.612X + 45.485 | 0.9998 | 50.0–500.0 | 7.50 | 20.00 | 1.6 | 99.2 | 1.2 | 1.9 |
Remifentanil | Y = 18.174X−0.3289 | 0.9996 | 0.5–10.0 | 0.07 | 0.20 | 1.2 | 99.4 | 1.3 | 1.6 |
Butorphanol | Y = 8.4406X + 13.256 | 0.9993 | 10.0–100.0 | 0.20 | 0.80 | 0.8 | 99.5 | 0.9 | 1.7 |
Bupivacaine | Y = 1.4093X + 7.1182 | 0.9992 | 50.0–500.0 | 2.00 | 6.00 | 1.0 | 101.1 | 1.3 | 2.0 |
Droperidol | Y = 9.9134X + 0.8142 | 0.9994 | 5.0–50.0 | 0.10 | 0.25 | 0.8 | 101.6 | 0.4 | 1.0 |
Fentanyl | Y = 70.895X−7.5161 | 0.9994 | 2.5–50.0 | 0.20 | 0.70 | 1.3 | 101.5 | 1.1 | 1.4 |
Lornoxicam | Y = 5.4772X−4.4635 | 0.9992 | 16.0–160.0 | 0.80 | 2.00 | 0.5 | 98.9 | 1.0 | 1.8 |
Sufentanil | Y = 63.32X−4.9491 | 0.9995 | 0.5–10.0 | 0.03 | 0.10 | 1.8 | 98.8 | 1.3 | 1.8 |