Table 2 System suitability parameters of the HPLC–DAD method
Analyte name | Retention time (tR) | Asymmetry factor (A) | Capacity factor (K) | Resolution factor (Rs) | Selectivity factor (α) | Number of theoretical plates (EP) | HETP (mm) |
|---|---|---|---|---|---|---|---|
Morphine | 4.85 | 1.39 | 0.94 | 5.47 | 1.43 | 10,655 | 0.0141 |
Hydromorphone | 5.86 | 0.80 | 1.34 | 11.17 | 1.60 | 17,117 | 0.0088 |
Oxycodone | 7.88 | 1.03 | 2.15 | 15.06 | 1.73 | 28,979 | 0.0052 |
Ketamine | 11.79 | 1.20 | 3.72 | 8.31 | 1.36 | 19,782 | 0.0076 |
Tramadol | 15.11 | 1.18 | 5.04 | 2.38 | 1.09 | 16,919 | 0.0089 |
Dezocine | 16.25 | 1.09 | 5.50 | 7.89 | 1.22 | 17,245 | 0.0087 |
Ropivacaine | 19.27 | 1.09 | 6.71 | 3.54 | 1.05 | 78,738 | 0.0019 |
Remifentanil | 20.06 | 1.10 | 7.02 | 3.85 | 1.03 | 225,103 | 0.0007 |
Butorphanol | 20.67 | 1.11 | 7.27 | 4.57 | 1.04 | 302,207 | 0.0005 |
Bupivacaine | 21.37 | 1.07 | 7.55 | 2.29 | 1.02 | 304,259 | 0.0005 |
Droperidol | 21.71 | 1.12 | 7.69 | 3.98 | 1.03 | 350,326 | 0.0004 |
Fentanyl | 22.36 | 1.13 | 7.94 | 3.85 | 1.04 | 243,479 | 0.0006 |
Lornoxicam | 23.11 | 1.17 | 8.24 | 9.48 | 1.12 | 194,541 | 0.0007 |
Sufentanil | 25.53 | 1.15 | 9.21 | 2.64 | 1.04 | 114,393 | 0.0013 |