Table 3 Experimental matrices for designs based on variables study in two levels employed for evaluation of the bergenin MAE employing EtOH:H2O (6:4)
Experiment | T (°C) | t (min) | Root mass (mg) | Extract (mg) | % Bergenin yielda |
|---|---|---|---|---|---|
1 | − | − | 20.3 | 1.7 | 2.22 |
2 | − | + | 20.5 | 1.8 | 1.96 |
3 | + | − | 20.2 | 1.8 | 2.93 |
4 | + | + | 20.4 | 1.1 | 5.13 |
5 | 0 | 0 | 20.4 | 2.5 | 1.46 |
6 | 0 | 0 | 20.1 | 2.6 | 1.31 |
7 | 0 | 0 | 20.5 | 2.8 | 1.79 |