Parameter | Amount taken (μg/mL) | Proposed method | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Amount found (μg/mL) | % Found | Amount taken (μg/mL) | %Found | ||||||||||||
CTZ | PPA | NMS | CTZ | PPA | NMS | CTZ | PPA | NMS | CTZ | PPA | NMS | CTZ | PPA | NMS | |
3rd Derivative method | 8.0 | 40.0 | 160.0 | 7.9 | 40.0 | 161.5 | 98.75 | 100.0 | 98.13 | 5.0 | 10.0 | 90.0 | 99.72 | 99.29 | 98.08 |
9.0 | 45.0 | 180.0 | 9.01 | 45.5 | 183.0 | 100.1 | 101.1 | 99.44 | 6.0 | 11.0 | 95.0 | 100.5 | 101.3 | 97.26 | |
10.0 | 50.0 | 200.0 | 9.9 | 50.1 | 202.0 | 99.00 | 100.2 | 98.2 | 7.0 | 12.0 | 100.0 | 99.80 | 99.41 | 99.5 | |
Mean | 99.29 | 100.4 | 98.6 | 100.0 | 99.99 | 98.28 | |||||||||
± S.D. | 0.72 | 0.14 | 0.74 | 0.46 | 1.12 | 0.46 | |||||||||
t | 1.48 | 1.17 | 0.397 | ||||||||||||
F | 2.85 | 1.05 | 2.36 | ||||||||||||
HPLC method | 5.0 | 25.0 | 100.0 | 4.97 | 25.10 | 101.05 | 99.32 | 100.4 | 101.1 | 5.0 | 10.0 | 90.0 | 99.72 | 99.29 | 98.08 |
5.5 | 27.5 | 110.0 | 5.57 | 27.3 | 121.05 | 101.2 | 99.3 | 100.9 | 6.0 | 11.0 | 95.0 | 100.5 | 101.3 | 97.26 | |
6.0 | 30.0 | 120.0 | 5.97 | 30.1 | 107.9 | 99.43 | 100.2 | 98.09 | 7.0 | 12.0 | 100.0 | 99.8 | 99.41 | 99.5 | |
Mean | 99.98 | 99.97 | 100.0 | 100.0 | 99.99 | 98.28 | |||||||||
± S.D. | 1.27 | 0.61 | 1.66 | 0.46 | 1.12 | 0.46 | |||||||||
t | 0.035 | 0.045 | 1.49 | ||||||||||||
F | 6.046 | 3.7 | 2.205 |