Parameter | Amount taken (μg/mL) | Proposed method | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Amount found (μg/mL) | % Found | Amount taken (μg/mL) | % Found | ||||||||||||
CTZ | PPA | NMS | CTZ | PPA | NMS | CTZ | PPA | NMS | CTZ | PPA | NMS | CTZ | PPA | NMS | |
3rd Derivative method | 8.0 | 40.0 | 160.0 | 7.8 | 40.0 | 157.0 | 97.5 | 100.0 | 98.13 | 5.00 | 10.0 | 15.0 | 99.18 | 99.77 | 101.3 |
9.0 | 45.0 | 180.0 | 8.8 | 45.0 | 180.5 | 98.89 | 100.0 | 100.3 | 5.50 | 11.0 | 16.5 | 100.3 | 100.9 | 100.5 | |
10.0 | 50.0 | 200.0 | 9.7 | 49.0 | 199.0 | 98.0 | 98.00 | 99.50 | 6.00 | 12.0 | 18.0 | 101.8 | 99.43 | 98.99 | |
12.0 | 24.0 | 36.0 | 12.2 | 23.5 | 40.0 | 101.7 | 97.92 | 100.0 | 8.00 | 8.00 | 8.00 | 98.09 | 99.81 | 99.49 | |
40.0 | 40.0 | 40.0 | 39.1 | 40.0 | 40.0 | 100.0 | 100.0 | 100.0 | 10.0 | 10.0 | 10.0 | 100.7 | 99.77 | 99.63 | |
Mean | 99.21 | 98.18 | 99.59 | 100.0 | 99.94 | 99.98 | |||||||||
± S.D. | 1.67 | 1.18 | 0.95 | 1.54 | 0.80 | 0.34 | |||||||||
t | 1.22 | 0.139 | 0.70 | ||||||||||||
F | 1.39 | 1.16 | 1.12 | ||||||||||||
HPLC method | 5.0 | 25.0 | 100.0 | 4.89 | 39.95 | 98.44 | 97.70 | 100.2 | 98.44 | 5.00 | 10.0 | 15.0 | 99.18 | 99.77 | 101.3 |
5.5 | 27.5 | 110.0 | 5.57 | 44.11 | 110.2 | 101.3 | 100.6 | 100.2 | 5.50 | 11.0 | 16.5 | 100.3 | 100.9 | 100.5 | |
6.0 | 30.0 | 120.0 | 6.02 | 50.60 | 121.05 | 100.4 | 99.45 | 100.9 | 6.00 | 12.0 | 18.0 | 101.8 | 99.43 | 98.99 | |
12.0 | 24.0 | 36.00 | 12.0 | 23.7 | 36.64 | 100.3 | 99.64 | 101.8 | 8.00 | 8.00 | 8.00 | 98.09 | 99.81 | 99.49 | |
40.0 | 40.0 | 40.00 | 39.9 | 39.5 | 39.69 | 99.98 | 99.98 | 99.25 | 10.0 | 10.0 | 10.0 | 100.7 | 99.77 | 99.63 | |
Mean | 99.91 | 99.97 | 100.1 | 100.0 | 99.94 | 99.98 | |||||||||
± S.D. | 0.55 | 0.53 | 1.06 | 1.57 | 0.80 | 0.34 | |||||||||
t | 0.14 | 0.122 | 0.24 | ||||||||||||
F | 1.16 | 1.51 | 2.1 |