Parameters | Intra-day | Inter-day | ||||
---|---|---|---|---|---|---|
\(\overline{\text{x}} \pm {\text{S}} . {\text{D}}\) | % RSD | % Error | \(\overline{\text{x}} \pm {\text{S}} . {\text{D}}\) | % RSD | % Error | |
3rd Derivative method | ||||||
CTZ (μg/mL) | ||||||
8 | 99.04 ± 1.04 | 1.05 | 0.61 | 100.05 ± 0.24 | 0.24 | 0.14 |
20 | 98.04 ± 0.45 | 0.46 | 0.27 | 98.8 ± 0.27 | 0.27 | 0.16 |
40 | 97.65 ± 0.53 | 0.54 | 0.31 | 98.93 ± 0.25 | 0.25 | 0.15 |
PPA (μg/mL) | ||||||
20 | 98.89 ± 1.27 | 1.29 | 0.74 | 99.08 ± 0.85 | 0.86 | 0.49 |
50 | 100.7 ± 1.85 | 1.84 | 1.06 | 99.99 ± 1.42 | 1.42 | 0.82 |
100 | 99.2 ± 1.83 | 1.85 | 1.07 | 100.59 ± 1.18 | 1.17 | 0.68 |
NMS (μg/mL) | ||||||
40 | 98.23 ± 0.77 | 0.79 | 0.45 | 99.27 ± 1.09 | 1.1 | 0.63 |
100 | 99.27 ± 1.22 | 1.32 | 0.71 | 100.6 ± 0.6 | 0.60 | 0.34 |
120 | 98.32 ± 0.62 | 0.63 | 0.36 | 99.91 ± 1.02 | 1.02 | 0.59 |
HPLC method | ||||||
CTZ (μg/mL) | ||||||
8 | 98.63 ± 0.95 | 0.96 | 0.56 | 100.53 ± 0.68 | 0.67 | 0.39 |
20 | 98.87 ± 0.49 | 0.50 | 0.29 | 100.75 ± 0.4 | 0.39 | 0.23 |
40 | 98.18 ± 0.47 | 0.48 | 0.27 | 98.15 ± 1.1 | 1.12 | 0.65 |
PPA (μg/mL) | ||||||
20 | 98.23 ± 0.55 | 0.56 | 0.32 | 99.53 ± 0.49 | 0.5 | 0.29 |
50 | 98.07 ± 0.15 | 0.16 | 0.09 | 99.88 ± 0.17 | 0.17 | 0.10 |
100 | 98.23 ± 0.83 | 0.83 | 0.49 | 98.94 ± 0.21 | 0.22 | 0.12 |
NMS (μg/mL) | ||||||
40 | 98.52 ± 0.62 | 0.63 | 0.36 | 98.77 ± 0.42 | 0.42 | 0.24 |
100 | 98.67 ± 0.36 | 0.36 | 0.21 | 99.45 ± 0.52 | 0.52 | 0.30 |
120 | 98.52 ± 0.95 | 0.96 | 0.55 | 100.24 ± 0.87 | 0.87 | 0.5 |