Compound | 3rd derivative method | HPLC method | |||||||
---|---|---|---|---|---|---|---|---|---|
Amount taken (μg/mL) | Amount found (μg/mL) | % Found | Amount taken (μg/mL) | Amount found (μg/mL) | % Found | Amount taken (μg/mL) | Amount found (μg/mL) | % Found | |
CTZ | 8.00 | 7.9 | 98.75 | 5.00 | 4.905 | 98.10 | 5.00 | 4.98 | 99.58 |
10.00 | 10.00 | 100.00 | 6.00 | 5.918 | 98.63 | 7.00 | 7.04 | 100.59 | |
16.00 | 16.9 | 99.38 | 8.00 | 8.036 | 100.45 | 9.00 | 8.98 | 99.77 | |
50.00 | 49.0 | 98.00 | 10.00 | 10.051 | 100.51 | ||||
60.00 | 59.9 | 99.83 | 20.00 | 20.172 | 100.86 | ||||
90.00 | 88.00 | 97.78 | 40.00 | 39.918 | 99.80 | ||||
Mean | 98.96 | 99.73 | 99.98 | ||||||
± S.D. | 0.94 | 0.44 | 0.58 | ||||||
t | 1.72 | 0.365 | |||||||
F | 3.04 | 4.36 | |||||||
PPA | 20.00 | 20.00 | 100.0 | 10.00 | 9.842 | 98.42 | 10.00 | 9.898 | 98.98 |
30.00 | 29.57 | 98.58 | 25.00 | 24.932 | 99.73 | 11.00 | 11.204 | 101.85 | |
40.00 | 39.5 | 98.75 | 30.00 | 30.334 | 101.11 | 12.00 | 11.898 | 99.15 | |
50.00 | 48.5 | 97.00 | 35.00 | 34.263 | 97.89 | ||||
80.00 | 78.5 | 98.13 | 50.00 | 50.877 | 101.75 | ||||
100.00 | 99.0 | 99.0 | 100.0 | 99.8 | 99.75 | ||||
Mean | 98.58 | 99.78 | 99.99 | ||||||
± S.D. | 1.23 | 1.49 | 1.61 | ||||||
t | 1.66 | 0.203 | |||||||
F | 2.60 | 1.17 | |||||||
NMS | 20.00 | 20.0 | 100.0 | 10.00 | 10.10 | 101.07 | 50.00 | 50.71 | 101.42 |
30.00 | 29.50 | 98.33 | 30.00 | 30.257 | 100.86 | 70.00 | 68.82 | 98.31 | |
40.00 | 39.00 | 97.50 | 40.00 | 40.254 | 100.64 | 100.00 | 100.47 | 100.47 | |
100.00 | 100.5 | 100.5 | 50.00 | 49.482 | 98.96 | ||||
180.00 | 179.50 | 99.72 | 100.00 | 99.048 | 99.05 | ||||
200.00 | 198.00 | 99.00 | 120.00 | 120.85 | 100.71 | ||||
Mean | 99.18 | 100.22 | 100.07 | ||||||
± S.D. | 1.28 | 1.1 | 1.53 | ||||||
t | 0.989 | 0.179 | |||||||
F | 2.02 | 2.8 |