Parameter | No. of theoretical plates (N) | Resolution (Rs) | Tailing factor (T) | Capacity factor (K’) | Selectivity factor (α) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTZ | PPA | NMS | CTZ/NMS | NMS/PPA | CTZ | PPA | NMS | CTZ | PPA | NMS | CTZ/NMS | NMS/PPA | |
PH of the mobile phase | |||||||||||||
3 | 1330 | 979 | 1947 | 1.2 | 3.8 | 1.26 | 1.39 | 1.25 | 2.1 | 0.33 | 1.5 | 1.4 | 4.5 |
4.6 | 1398 | 1246 | 1548 | 1.25 | 4.6 | 1.31 | 1.5 | 1.37 | 2.61 | 0.367 | 2.04 | 1.28 | 5.6 |
6 | 2351 | 1248 | 1490 | 2.1 | 4.68 | 1.30 | 1.35 | 1.30 | 3.47 | 0.74 | 2.56 | 1.36 | 3.45 |
7 | 2432 | 1794 | 2804 | 3.8 | 5.1 | 1.19 | 1.11 | 1.23 | 4.5 | 1.05 | 2.8 | 1.64 | 2.6 |
Conc. of phosphate buffer | |||||||||||||
0.05 | 1947 | 1696 | 2497 | 1.1 | 4.1 | 1. 34 | 1.49 | 1.38 | 3.4 | 1.06 | 2.75 | 1.24 | 2.5 |
0.1 | 2432 | 1794 | 2804 | 3.8 | 5.1 | 1. 19 | 1.11 | 1.23 | 4.5 | 1.05 | 2.8 | 1.64 | 2.6 |
0.2 | 1146 | 1280 | 1855 | 1.9 | 3.66 | 1.23 | 1.52 | 1.36 | 3.4 | 0.58 | 2.05 | 1.7 | 3.55 |
Conc. of methanol (% v/v) | |||||||||||||
50% | 1513 | 1309 | 2133 | 2.1 | 4.1 | 2.07 | 0.99 | 1.32 | 4.78 | 1.1 | 3.4 | 1.4 | 3.3 |
60% | 2432 | 1794 | 2804 | 3.8 | 5.1 | 1.19 | 1.11 | 1.23 | 4.5 | 1.05 | 2.8 | 1.64 | 2.6 |
70% | 2396 | 1271 | 1496 | 0.5 | 3.8 | 2.22 | 1.9 | 1.32 | 2.47 | 1.02 | 2.3 | 1.07 | 2.25 |
80% | 1638 | 1229 | 1369 | 0.74 | 2.1 | 1.23 | 1.09 | 1.33 | 1.86 | 0.99 | 1.62 | 1.15 | 1.64 |
Type of organic modifier | |||||||||||||
Methanol | 2432 | 1794 | 2804 | 3.8 | 5.1 | 1.19 | 1.11 | 1.23 | 4.5 | 1.05 | 2.8 | 1.64 | 2.6 |
Acetonitrile | 2278 | 1374 | 1795 | 2.1 | 4.1 | 1.36 | 0.77 | 1.27 | 3.2 | 0.5 | 2.03 | 1.59 | 4.6 |
n-Propanol | 1920 | 900 | 1058 | 2.4 | 3.9 | 3.22 | 1.9 | 2.3 | 2.88 | 0.42 | 1.88 | 1.5 | 4.5 |
Flow rate (mL/min) | |||||||||||||
0.8 | 1889 | 1123 | 2543 | 2.4 | 3.9 | 1.2 | 1.56 | 1.28 | 3.4 | 0.98 | 1.8 | 1.88 | 1.84 |
1.0 | 2432 | 1794 | 2804 | 3.8 | 5.1 | 1.19 | 1.11 | 1.23 | 4.5 | 1.05 | 2.8 | 1.64 | 2.6 |
1.2 | 2117 | 1247 | 2178 | 1.1 | 2.9 | 1.32 | 1.56 | 1.35 | 2.9 | 1.00 | 2.3 | 1.3 | 2.30 |