Parameter | 3rd Derivative method | HPLC method | ||||
---|---|---|---|---|---|---|
CTZ | PPA | NMS | CTZ | PPA | NMS | |
Linearity range (µg/mL) | 8–90 | 20–100 | 20–200 | 5–40 | 10–100 | 10–120 |
Intercept (a) | 0.006 | −0.028 | −0.036 | 1.3 × 104 | 4.926 × 105 | −7.217 × 104 |
Slope (b) | 0.001 | 0.002 | 0.002 | 4.2399 × 104 | 3.1 × 104 | 9.343 × 104 |
Correlation coefficient (r) | 0.9999 | 0.9999 | 0.9999 | 0.9999 | 0.9998 | 0.9999 |
S.D. of residuals (Sy/x) | 5.061 × 10−4 | 1.146 × 10−3 | 1.169 × 10−3 | 5.015 × 103 | 1.912 × 104 | 6.67 × 104 |
S.D. of intercept (Sa) | 3.371 × 10−4 | 1.16 × 10−3 | 1.143 × 10−3 | 3.21 × 103 | 1.377 × 104 | 4.908 × 104 |
S.D. of slope (Sb) | 6.828 × 10−6 | 1.794 × 10−5 | 9.583 × 10−6 | 1.667 × 102 | 2.723 × 102 | 7.00 × 102 |
S.D. | 0.94 | 1.51 | 1.28 | 0.44 | 1.49 | 1.10 |
% RSDa | 0.95 | 1.53 | 1.29 | 0.44 | 1.49 | 1.10 |
% Errorb | 0.39 | 0.86 | 0.53 | 0.18 | 0.61 | 0.45 |
LOD (µg/mL)c | 1.10 | 1.90 | 1.90 | 0.25 | 1.47 | 1.70 |
LOQ (µg/mL)d | 3.40 | 5.80 | 5.50 | 0.76 | 4.40 | 5.25 |