Table 3 Stability of the yakuchinone A and IS in rat plasma (n = 5)
Peak area (× 104) | ||||||
|---|---|---|---|---|---|---|
Short-term stability (4 h at room temperature) | Autosampler stability (8 h at room temperature) | Freeze-thaw stability (3 cycles) | ||||
Mean ± SD | RSD | Mean ± SD | RSD | Mean ± SD | RSD | |
Yakuchinone A | ||||||
4 | 5.04 ± 0.18 | 3.59% | 5.61 ± 0.21 | 3.72% | 5.18 ± 0.13 | 2.60% |
80 | 79.7 ± 1.8 | 1.49% | 96.4 ± 1.9 | 2.01% | 80.4 ± 7.9 | 9.84% |
800 | 829 ± 82 | 9.86% | 834 ± 18 | 2.22% | 801 ± 106 | 13.2% |
IS | ||||||
80 | 49.5 ± 1.5 | 3.04% | 51.1 ± 1.7 | 3.35% | 45.7 ± 4.5 | 9.76% |