Table 2 Matrix effect and extraction recovery for the yakuchinone A and IS in rat plasma (n = 5)
Nominal conc. (ng/ml) | Peak area (× 104) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Set 1 | Set 2 | Set 3 | Matrix effect | Extraction efficiency | ||||||
Mean ± SD | RSD | Mean ± SD | RSD | Mean ± SD | RSD | Mean (%) | RSD (%) | Mean (%) | RSD (%) | |
Yakuchinone A | ||||||||||
4 | 7.45 ± 0.15 | 2.04% | 6.43 ± 0.04 | 0.70% | 5.18 ± 0.12 | 2.28% | 86.3 | 1.95 | 80.5 | 2.30 |
80 | 113 ± 6 | 5.05% | 111 ± 3 | 2.59% | 79.9 ± 1.2 | 1.56% | 98.5 | 6.95 | 71.7 | 2.89 |
800 | 930 ± 61 | 6.56% | 884 ± 19 | 2.11% | 789 ± 12 | 1.50% | 95.5 | 8.54 | 89.2 | 1.19 |
IS | ||||||||||
80 | 80.7 ± 7.8 | 9.65% | 89.7 ± 1.9 | 2.16% | 52.7 ± 2.5 | 4.66% | 112 | 9.30 | 58.8 | 3.68 |