Application of smart spectrophotometric methods and artificial neural network for the simultaneous quantitation of olmesartan medoxamil, amlodipine besylate and hydrochlorothiazide in their combined pharmaceutical dosage form

Table 3 Assay validation sheet of the proposed methods for the simultaneous determination of AML, OLM and HCT

Parameter	Spectrophotometric methods			ANN method
	Direct	Isoabsorptive	Ratio subtraction	ANN method
	AML	OLM	HCT	AML	OLM	HCT
Accuracy (mean ± RSD)	99.89 ± 0.937	101.96 ± 0.622	99.55 ± 1.889	100.47 ± 1.197	99.9 ± 1.066	100.75 ± 2.072
Repeatability^a	0.552	0.829	0.587	1.321	1.061	1.144
Intermediate precision^b	0.925	1.327	1.593	1.119	1.683	2.424
Linear range (μg mL^-1)	5-40	2.5-40	5-40	3-40	2.5-40	5-40
Slope	0.011	0.041	0.010	1.012	0.976	0.998
Intercept	0.003	0.077	0.01	−0.035	0.390	0.041
Correlation coefficient (r)	0.9999	0.9989	0.9999	0.9992	0.9995	0.9995
LOD (μg mL^-1)	1.278	0.729	0.819	0.291	0.683	0.444
LOQ (μg mL^-1)	4.217	2.209	2.482	0.880	2.070	1.345

^a The intraday (n = 3), average of concentrations; 6.95,20,12.5 μg mL^-1for AML, OLM and HCT respectively, repeated three times within the day.
^b The interday (n = 3), average of concentrations; 6.95,20,12.5 μg mL^-1for AML, OLM and HCT respectively, repeated three times in three days.

ISSN: 2661-801X