Figure 1
Anti-proliferative effect of Mallotus phillipensis extracts. HL-60 cells were seeded into T-25 tissue culture flasks (1 × 105 cells/ml), grown for 24 h to enter logarithmic growth phase, and incubated with increasing concentrations (a) hexane extract (1.00, 1.50, and 2.00 mg dry roots equivalent/ml medium); (b) EtOAc extract (1.00, 1.50 and 2.0 mg dry roots equivalent/ml medium); (c) BuOH extract (1.00, 1.5 and 2.0 mg dry roots equivalent/ml). Cells were counted after 24, 48 and 72 h of treatment (white, light gray and dark gray columns, respectively) and the percentage of proliferation was calculated and compared to DMSO-controls (Control). Controls were considered as cells with a maximal proliferation rate (100%). Experiments were done in triplicate. Error bars indicate SEM, asterisks significance (p < 0.05).