Table 2 Optimum gradient elution conditions
Time (min) | Flow rate (mL/min) | %A (MeOH) | %B (Buffer pH 3.0) |
|---|---|---|---|
0.0 | 0.4 | 20.0 | 80.0 |
4.0 | 0.4 | 45.0 | 55.0 |
6.0 | 0.4 | 45.0 | 55.0 |
7.0 | 0.4 | 50.0 | 50.0 |
9.0 | 0.4 | 50.0 | 50.0 |
10.0 | 0.4 | 25.0 | 75.0 |
12.0 | 0.4 | 25.0 | 75.0 |
14.0 | 0.4 | 90.0 | 10.0 |
15.0 | 0.3 | 100.0 | 0.0 |
30.0 | 0.3 | 100.0 | 0.0 |
33.0 | 0.4 | 20.0 | 80.0 |
38.0 | 0.4 | 20.0 | 80.0 |