Table 10 The Analytical eco-scale penalty points of the developed RP-HPLC-PDA method
Parameters | Amount | Penalty points | |
|---|---|---|---|
Reagents | |||
Methanol | More than 100 mL | 18 | |
Phosphate buffer | Less than 10 mL | 2 | |
Energy consumption | Less than or equal to 0.1kWh per sample | 0 | |
Occupational Hazard | None | 0 | |
Waste | 1–10 mL | 3 | |
Total penalty points | 23 | ||
Eco-scale out of 100 | 77 | ||
Greenness of the method | Excellent green analysis | ||