Simultaneous determination of bromoxynil and MCPA in commercial samples and raw materials using reversed phase high performance liquid chromatography

Table 8 Summary of Validation Parameters for Bromoxynil

Parameters		Results (Bromoxynil)		Acceptance limit
Linearity		Correlation Coefficient = 0.992		Correlation Coefficient NLT^a 0.97
Precision		0.06% RSD		% RSD NMT^b 2.0
Trueness		Conc. (mg/L)	% Recovered	% Recovery within 80–120%
		150	99.6%
		200	99.8%
		250	99.7%
		300	99.7%
		350	99.6%
Repeatability	(with respect to Instrument and Analyst)	0.13% RSD		RSD ≤ 2.0%
Reproducibility	(with respect to Instrument)	HPLC–20AT	HPLC–10AT
	(with respect to Instrument)	0.21% RSD	0.13% RSD
	[with respect to various Labs (ILC)]	Average 0.18%RSD
Detection and quantitation limit		LOD	LOQ	−
Detection and quantitation limit		1.57 mg/L	5.22 mg/L	−
Robustness		Change	% RSD	% RSD NMT 1.5
		Flow rate = 1.3 mL	0.10%
		Flow rate = 1.5 mL	0.13%
		Flow rate = 1.7 mL	0.05%
		(Mobile Phase) Methanol: Water 950: 50	0.24%
		(Mobile Phase) Methanol: Water 900: 100	0.13%
		(Mobile Phase) Methanol: Water 850: 150	0.13%

^aNot Less than in accordance to the ICH Analytical procedures developments Guidelines [43]
^bNot More than in accordance to the ICH Analytical procedures developments Guidelines [43]

ISSN: 2661-801X