(a) Robustness of the developed method at the change of flow rate and mobile phase for the Bromoxynil | ||||||
---|---|---|---|---|---|---|
Sample No | Change of Flow Rate | Change of Mobile Phase | ||||
Peak area at 1.3 mL/min | Peak area at 1.5 mL/min | Peak area at 1.7 mL/min | Methanol: Water 95: 05 | Methanol: Water 90: 10 | Methanol: Water 85: 15 | |
01 | 16899261 | 14648612 | 13095952 | 11794631 | 14648612 | 19704492 |
02 | 16893790 | 14660667 | 13112501 | 11793598 | 14660667 | 19731947 |
03 | 16880153 | 14661086 | 13109821 | 11759541 | 14661086 | 19757886 |
04 | 16901669 | 14651265 | 13109991 | 11777437 | 14651265 | 19760224 |
05 | 16927612 | 14615510 | 13108926 | 11726960 | 14615510 | 19762280 |
Mean | 16900497 | 14647428 | 13107438 | 11770433 | 14647428 | 19743366 |
Standard deviation | 17299 | 18687 | 6557 | 28195 | 18687 | 24972 |
% RSD | 0.10% | 0.13% | 0.05% | 0.24% | 0.13% | 0.13% |
(b) Robustness of the developed method at the change of flow rate and mobile phase for the MCPA | ||||||
---|---|---|---|---|---|---|
Sample No | Change of Flow Rate | Change of Mobile Phase | ||||
Peak area at 1.3 mL/min | Peak area at 1.5 mL/min | Peak area at 1.7 mL/min | Methanol: Water 95: 05 | Methanol: Water 90: 10 | Methanol: Water 85: 15 | |
01 | 14290800 | 12455385 | 11060939 | 9951291 | 12455385 | 16818457 |
02 | 14279376 | 12380559 | 11058719 | 9950908 | 12380559 | 16843060 |
03 | 14267751 | 12381573 | 11057852 | 9921681 | 12381573 | 16875223 |
04 | 14294707 | 12469138 | 11051754 | 9910312 | 12469138 | 16883146 |
05 | 14342259 | 12459192 | 11047533 | 9899583 | 12459192 | 16904524 |
Mean | 14294979 | 12429169 | 11055359 | 9926755 | 12429169 | 16864882 |
Standard deviation | 28451 | 44200 | 5544 | 23557 | 44200 | 34072 |
% RSD | 0.20% | 0.36% | 0.05% | 0.24% | 0.36% | 0.20% |