HPLC estimation of iothalamate to measure glomerular filtration rate in humans

BMC Chemistry

Table 2 Summary of assay validation results

Analyte (µg/mL)	QC	Linear range	LOD	R²	Intra-day (N = 6)		Inter-day (N = 12)		Recovery
Analyte (µg/mL)	QC	Linear range	LOD	R²	Precision, % CV	Accuracy %	Precision, % CV	Accuracy %	Recovery
Iothalamic acid in plasma	1	1–150	0.5	0.9985	4.56	86.33	13.16	90.08
	2				9.46	112.5	12.87	102.58
	20				14.96	100.74	11.52	96.6	96.05
	120				2.66	99.88	2.65	98.99	118.38
Iothalamic acid in urine	25	25–1500	5	0.9992	4.31	97.54	4.45	95.91
	40				3.96	88.49	3.86	90.91
	200				2.23	114.96	6.68	108.33	93.14
	1200				1.81	109.8	8.56	101.66	111.74

These values were well within the assay criteria for accuracy of nominal concentration ±15%

ISSN: 2661-801X