Table 1 Fatty acids composition of the forage from the 10 trials with different fertilization, using BF3/MeOH and HCl/MeOH methods (average contents of 3 different samples for each trial; significance: ns = p > 0.05, *** = p < 0.001)
| Â | Fatty acids, mg kg -1 | Significance | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
FA/Fertilized trials | Lauric (12:0) | Myristic (14:0) | Palmitic (16:0) | Stearic (18:0) | Oleic (18:1n9) | Linoleic (18:2n6) | Linolenic (18:3n3) | Arahidic (20:0) | Behenic (22:0) | FAs Sum | A (methods) | B (FAs) |
BF 3 /MeOH method | ||||||||||||
D1 | 33.4 | 73.7 | 1644.4 | 237.1 | 465.8 | 1167.9 | 665.7 | 132.2 | 129.2 | 4549.4 | ns | *** |
D2 | 19.1 | 55.2 | 1876.3 | 239.9 | 564.7 | 1712.3 | 1605.9 | 127.0 | 103.9 | 6304.3 | ns | *** |
D3 | 23.3 | 79.0 | 2257.6 | 424.1 | 736.8 | 2681.0 | 1546.9 | 169.4 | 154.6 | 8072.7 | ns | *** |
D4 | 18.6 | 65.0 | 2413.7 | 509.8 | 851.8 | 2909.5 | 1988.1 | 207.5 | 150.5 | 9114.4 | ns | *** |
D5 | 15.6 | 47.8 | 1729.9 | 295.8 | 565.0 | 1929.3 | 757.0 | 202.1 | 122.2 | 5664.7 | ns | *** |
D6 | 15.3 | 51.4 | 1764.1 | 282.9 | 513.5 | 1471.4 | 749.4 | 125.1 | 76.3 | 5049.5 | ns | *** |
D7 | 24.0 | 59.2 | 1474.4 | 200.3 | 423.2 | 1027.3 | 615.4 | 124.4 | 105.8 | 4053.9 | ns | *** |
D8 | 18.4 | 55.9 | 1535.4 | 189.4 | 441.7 | 1069.6 | 608.2 | 106.8 | 96.0 | 4121.4 | ns | *** |
D9 | 20.6 | 52.8 | 1531.5 | 179.0 | 352.2 | 721.6 | 509.7 | 93.2 | 77.5 | 3538.1 | ns | *** |
D10 | 18.4 | 46.2 | 1378.7 | 156.4 | 298.4 | 673.4 | 475.0 | 104.2 | 94.2 | 3245.0 | ns | *** |
Average SDV | 1.9 | 3.2 | 61.4 | 14.7 | 15.0 | 10.7 | 7.9 | 6.6 | 13.3 | 131.4 | Â | Â |
Average RSDV % | 25.5 | 9.9 | 4.3 | 7.8 | 6.2 | 1.9 | 1.4 | 7.4 | 12.2 | 4.0 | Â | Â |
HCl/MeOH method | ||||||||||||
D1 | 14.2 | 43.1 | 1642.0 | 236.7 | 577.5 | 1349.6 | 523.8 | 142.0 | 70.5 | 4599.4 | ns | *** |
D2 | 8.8 | 17.6 | 1805.7 | 232.3 | 620.9 | 1673.7 | 1310.5 | 115.1 | 77.1 | 5861.6 | ns | *** |
D3 | 2.8 | 13.5 | 2208.5 | 518.3 | 974.6 | 2781.7 | 1223.9 | 131.0 | 156.3 | 8010.7 | ns | *** |
D4 | 4.8 | 44.2 | 2622.6 | 542.2 | 970.9 | 3252.0 | 1808.1 | 230.5 | 238.9 | 9714.2 | ns | *** |
D5 | 9.6 | 12.4 | 1876.8 | 341.9 | 782.5 | 2175.4 | 728.3 | 252.8 | 160.1 | 6339.9 | ns | *** |
D6 | 4.1 | 26.9 | 1731.4 | 271.3 | 534.3 | 1388.7 | 612.0 | 136.7 | 118.1 | 4823.6 | ns | *** |
D7 | 5.4 | 24.1 | 1449.8 | 178.2 | 438.1 | 1146.9 | 489.1 | 108.5 | 105.9 | 3946.0 | ns | *** |
D8 | 12.3 | 42.5 | 1674.6 | 223.7 | 499.4 | 1241.3 | 536.2 | 124.6 | 135.9 | 4490.6 | ns | *** |
D9 | 15.1 | 38.8 | 1401.4 | 188.6 | 328.8 | 721.2 | 423.6 | 67.9 | 108.9 | 3294.3 | ns | *** |
D10 | 8.2 | 49.3 | 1467.6 | 186.9 | 444.7 | 805.2 | 402.6 | 100.7 | 119.1 | 3584.4 | ns | *** |
Average SDV | 4.9 | 4.2 | 218.1 | 72.2 | 189.1 | 115.9 | 68.6 | 6.7 | 13.5 | 669.4 | Â | Â |
Average RSDV% | 130.6 | 18.0 | 13.2 | 29.5 | 54.3 | 17.9 | 11.6 | 7.7 | 13.9 | 18.1 | Â | Â |