Stress degradation studies and development of stability-indicating TLC-densitometry method for determination of prednisolone acetate and chloramphenicol in their individual and combined pharmaceutical formulations

Table 4 Summary of stress degradation studies of prednisolone acetate and chloramphenicol in combination.

Degradation conditions	% Degradation of 1	% Degradation of 2	R_f of degraded products
Acidic hydrolysis^a
1N HCl	100	31.8	0.01, 0.04, 0.17, 0.29,0.78, 0.81
5N HCl	100	82.7	0.01, 0.02, 0.04, 0.17, 0.29, 0.38,0.58, 0.78
Basic hydrolysis^a
0.1 N NaOH	100	100	0.01, 0.02, 0.17,0.29, 0.50, 0.79,0.85
1N NaOH	100	100	0.01, 0.02, 0.03, 0.29,0.44
5N NaOH	100	100	0.01, 0.02, 0.31
Neutral hydrolysis^a
H₂O	74.52	17.9	0.01, 0.17, 0.29, 0.79
Wet heating^a	17	36.9	0.01, 0.17, 0.29, 0.50,0.79
Dry heating	0	0	0.01, 0.26, 0.47,0.73,0.79
Photochemical	85.808	17.1	0.01, 0.47, 0.49,0.50,0.58, 0.69,0.73
Oxidation
35%v/v H₂O₂ ^a	30.56	7.20	0.01, 0.12, 0.31, 0.55
At room temp	9.029	13.4	0.01, 0.12, 0.23,0.31, 0.55

ISSN: 2661-801X