Table 3 Method validation results of the target compounds
From: Rapid determination of natural steroidal hormones in saliva for the clinical diagnoses
Compound | Retention Time (min) | Calibration curve | Precision and Accuracy (n = 5) | Recovery (%) (n = 5) | |||
|---|---|---|---|---|---|---|---|
Linear Equation | R2 | Spiked Conc (μg L-1) | Accuracy (%) | Precision (%) | |||
bis-TMS-E1 | 11.29 | y = 0.0707x - 0.0001 | 0.9982 | 0.05 | 100 | 7.18 | 104 ± 7.4 |
0.10 | 112 | 5.62 | 105 ± 5.9 | ||||
bis-TMS-E2 | 11.42 | y = 0.0768x + 0.0003 | 0.9979 | 0.05 | 104 | 10.8 | 93.0 ± 10.0 |
0.10 | 101 | 4.74 | 106 ± 5.0 | ||||
tri-TMS-E3 | 12.70 | y = 0.3431x + 0.0002 | 0.9986 | 5.0 | 112 | 8.75 | 111 ± 8.7 |
10.0 | 104 | 8.86 | 94.4 ± 7.9 | ||||
TMS-P | 12.79 | y = 0.0315x + 0.0003 | 0.9986 | 0.05 | 108 | 6.08 | 98.6 ± 6.0 |
0.10 | 96.0 | 3.12 | 105 ± 3.3 | ||||
bis-TMS-T | 11.45 | y = 0.1738x + 0.0037 | 0.9986 | 0.05 | 108 | 6.76 | 101 ± 4.2 |
0.10 | 97.0 | 6.00 | 108 ± 4.8 | ||||