Sub-strate | Kinetic parameter | Dye | |||||
---|---|---|---|---|---|---|---|
ARS | PNS | XO | MB | MG | MO | ||
Reducing substrate | K M (mM) | 2.11 ± 0.74 | 0.32 ± 0.04 | 1.33 ± 0.38 | 0.11 ± 0.02 | 0.72 ± 0.12 | 0.63 ± 0.05 |
kcat (min-1) | 0.14 ± 0.03 | 2.2 ± 0.1 | 98 ± 14 | 19.0 ± 1.8 | 108 ± 9 | 67 ± 4 | |
kcat/K M (mM-1 min-1) | 0.066 ± 0.037 | 7.0 ± 1.2 | 74 ± 31 | 166 ± 52 | 150 ± 37 | 105 ± 16 | |
H 2 O 2 | K M (mM) | 3.21 ± 0.12 | 3.0 ± 0.4 | 7.9 ± 1.9 | 2.4 ± 0.5 | 0.43 ± 0.08 | 7.4 ± 1.5 |
kcat (min-1) | 0.13 ± 0.01 | 1.9 ± 0.1 | 78 ± 8.9 | 15.2 ± 1.3 | 159 ± 12 | 71 ± 8 | |
kcat/K M (mM-1 min-1) | 0.040 ± 0.004 | 0.63 ± 0.13 | 9.9 ± 3.6 | 6.4 ± 2.1 | 369 ± 100 | 9.6 ± 3.2 |