- Research article
- Open Access
Biosynthesis of silver nanoparticles using Artocarpus elasticus stem bark extract
© Abdullah et al. 2015
Received: 3 February 2015
Accepted: 22 September 2015
Published: 2 November 2015
Green approach in synthesizing metal nanoparticles has gain new interest from the researchers as metal nanoparticles were widely applied in medical equipment and household products. The use of plants in the synthesis of nanoparticles emerges as a cost effective and eco-friendly approach. A green synthetic route for the production of stable silver nanoparticles (Ag-NPs) by using aqueous silver nitrate as metal precursor and Artocarpus elasticus stem bark extract act both as reductant and stabilizer is being reported for the first time.
The resultant Ag-NPs were characterized by UV–vis spectroscopy, powder X-Ray diffraction, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Fourier-transform infra-red (FT-IR). The morphological study by TEM and SEM shows resultant Ag-NPs in spherical form with an average size of 5.81 ± 3.80, 6.95 ± 5.50, 12.39 ± 9.51, and 19.74 ± 9.70 nm at 3, 6, 24, and 48 h. Powder X-ray diffraction showed that the particles are crystalline in nature, with a face-centered cubic structure. The FT-IR spectrum shows prominent peaks appeared corresponds to different functional groups involved in synthesizing Ag-NPs.
Nanotechnology has been emerged as a new technology which design, characterize, produce and applied in the structures, devices and systems by controlling the shape and size at nanometer scale, range from 100 nm down to 1 nm .
Metal nanoparticles that have high interest to be synthesized are Ag, Au, Pt and Pb. Silver nanoparticles (Ag-NPs) have the least toxicity to animal cells and highest toxicity to microorganism cells compared to the other metals . Various works have been reported on toxicity of silver nanoparticle against micro-organism such as bacteria , fungi , viruses , and also larvicidal activity . Silver has been widely used in household products such as paint , cotton fabrics , and in water purification . It was also been applied in surface enhanced raman spectroscopy , optical sensor , catalyst  and in biomedical application .
Metal nanoparticles have been synthesized in various techniques in reducing the silver into Ag-NPs including conventional chemical reduction , electrochemical , irradiation [16, 17], laser ablation , polysaccharide . Synthesis of metallic nanoparticles by using living organism is the new approach towards green technology, denominate as biosynthesis.
Biosynthesis of metal nanoparticles includes algae , bacteria , fungi , yeast , actinomycetes , and plants . From the plant itself, various parts have been explored to give different properties of Ag-NPs. It includes leaf, stem bark, root, flower, vegetable oil, fruit, peel, leaf bud, seed, and callus [26–28]. In addition, biosynthetic process is clearly abiding the three rules of green principles compared to conventional method of chemical reduction.
The Artocarpus elasticus (A. elasticus) is a distinctive tree in nature, easy to grow, possess anticancer [29, 30], and antimalarial properties . Locals have been using the leaves to nursing mothers, young shoots in curing vomiting blood problems, inner bark used in treating ulcers, and its latex used for dysentery disease . Artocarpus are sources of phenolic-derived secondary metabolites which includes flavonoid compounds, particularly of prenylated flavones that exist as the main group of the phenolic constituents . Some of the compounds that have been isolated were artelastin, artelastochromene, artelasticin and artocarpesin .
To the best of our knowledge, there is no work reported on Ag-NPs or any other metal nanoparticles synthesized by using A. elasticus at ambient temperature. Here, we demonstrate the biosynthesis and characterization of Ag/A. elasticus nanoparticles by using silver nitrate and stem bark extract of A. elasticus.
Results and discussion
After dispersion of silver ions in the A. elasticus aqueous solution matrix (Eq. 1), the extract was reacted with the Ag+ (aq) to form [Ag/A. elasticus)]+ complex, which reacted with functional groups of A. elasticus components to form [Ag/A. elasticus)] (Eq. 2) after left stirred for 48 h [35, 36].
UV–visible spectroscopy analysis
At 1 h of reaction time, low intensity of maximum SPR was recorded at 406 nm. However, with increasing time, particles aggregates, causing the conduction electrons near each particle surface become delocalized and shared among neighbouring particles, thus red-shifting the SPR into longer wavelengths from 406 to 424, 420, 433, 455 and 460 nm. At the end of the reaction (48 h), the absorbance was considerably increased and the λmax value was slightly red-shifted to 460 nm, compared with the 24 h reaction time.
At the initial stage of the reaction, the Ag-NPs formed with a narrow size distribution which led to a SPR peak at about 406 nm. After this stage, the Ag-NPs could associate due to increases of reaction time to form bigger size of Ag-NPs. However, at 48 h of reaction time, the absorbance is the largest but also broad compared to the other reaction time, suggesting bigger silver nanoparticles with stable properties. Shoulder peaks were also observed for all of the samples, at 350 nm , indicating the existence of bulk silver. Other works presented a broader peak with maximum at 490 nm that indicating larger size of Ag-NPs . However, at 72 h of reaction time, the particles agglomerate, thus showing no distinguishable maximum SPR band. After reaching certain particle size, the plant extract which act as stabilizer was no longer able to withhold the nanoparticles from agglomeration .
Powder X-ray diffraction
FT-IR chemical analysis
FT-IR measurements were carried out to identify the possible biomolecules responsible for the reduction; capping and stabilization of the Ag-NPs synthesized using A. elasticus extract. For this analysis, solvent was removed to produce Ag/A. elasticus nanoparticles powder in order to remove unbound components.
The A. elasticus stem barks were collected from Terengganu, Malaysia. Silver nitrate (99.98 %) was purchased from Merck, Germany and used as silver precursor. All reagents used were of analytical grade. All aqueous solutions were prepared using distilled water. All glassware used were cleaned and washed with distilled water and dried before used.
The air-dried stem bark was ground into fine powder. The fine powder (400 g) was extracted with 2500 ml of methanol/water overnight at ratio of 70:30 at room temperature. The solution was then filtered; the residue was collected and re-extracted. The solvent then was removed by using rotary vacuum evaporator under vacuum. The concentrated extract was then kept in dark at 4 °C until used.
Synthesis of Ag/A. elasticus nanoparticles
0.5 g of A. elasticus was added into 0.01 M aqueous solution of AgNO3 (100 ml) with constant stirring at room temperature. Ag-NPs were obtained during the incubation period of 1, 3, 6, 12, 24 and 48 h. Colour changes from light brown to dark brown due to excitation of surface plasmon resonance were observed. The Ag/A. elasticus nanoparticles emulsion obtained were kept at 4 °C.
Characterization methods and instruments
The prepared Ag/A. elasticus nanoparticles were characterized by UV–visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Fourier-transform infrared (FT-IR) spectroscopy. The reduction of silver ions was confirmed by measuring the UV–vis spectrum at 300–700 nm range with UV-1601 Shimadzu, in a glass cuvette. The structures of the Ag-NPs synthesized after 48 h of incubation were examined with using XRD in powder diffractometer, drop coated onto glass substrates. TEM observation of the Ag-NPs prepared was carried out with LEO 912AB EFTEM. The Ag/A. elasticus nanoparticle solutions were drop onto copper grid and were analyzed. Morphological characterization of the Ag/A. elasticus nanoparticles was performed by Scanning Electron Microscope with using Jeol JSM-7600F Field Emission SEM. The dried powder of Ag/A. elasticus nanoparticles were coated on a carbon tape and coated again with gold before subjected to analysis. The FT-IR spectra were recorded in the range of 280-4000 cm−1 using FT-IR Perkin-Elmer.
NISA carried out the synthesis, and the characterization of the compounds. NISA and KS carried out the acquisition of data, analysis and interpretation of data collected and involved in drafting of manuscript. MA and KS involved in revision of draft for important intellectual content and give final approval of the version to be published. All authors read and approved final manuscript.
The authors were gratitude by University Putra Malaysia (UPM) for its facilities and equipment supports. The authors are also grateful to the staff of the Department of Chemistry UPM for their help in this research, Institute of Bioscience (IBS/UPM) for technical assistance.
The authors declare that they have no competing interests.
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