A disposable biosensor based on immobilization of laccase with silica spheres on the MWCNTs-doped screen-printed electrode
© Li et al.; licensee Chemistry Central Ltd. 2012
Received: 18 July 2012
Accepted: 3 September 2012
Published: 17 September 2012
Biosensors have attracted increasing attention as reliable analytical instruments in in situ monitoring of public health and environmental pollution. For enzyme-based biosensors, the stabilization of enzymatic activity on the biological recognition element is of great importance. It is generally acknowledged that an effective immobilization technique is a key step to achieve the construction quality of biosensors.
A novel disposable biosensor was constructed by immobilizing laccase (Lac) with silica spheres on the surface of multi-walled carbon nanotubes (MWCNTs)-doped screen-printed electrode (SPE). Then, it was characterized in morphology and electrochemical properties by scanning electron microscopy (SEM) and cyclic voltammetry (CV). The characterization results indicated that a high loading of Lac and a good electrocatalytic activity could be obtained, attributing to the porous structure, large specific area and good biocompatibility of silica spheres and MWCNTs. Furthermore, the electrochemical sensing properties of the constructed biosensor were investigated by choosing dopamine (DA) as the typical model of phenolic compounds. It was shown that the biosensor displays a good linearity in the range from 1.3 to 85.5 μM with a detection limit of 0.42 μM (S/N = 3), and the Michaelis-Menten constant (Kmapp) was calculated to be 3.78 μM.
The immobilization of Lac was successfully achieved with silica spheres to construct a disposable biosensor on the MWCNTs-doped SPE (MWCNTs/SPE). This biosensor could determine DA based on a non-oxidative mechanism in a rapid, selective and sensitive way. Besides, the developed biosensor could retain high enzymatic activity and possess good stability without cross-linking reagents. The proposed immobilization approach and the constructed biosensor offer a great potential for the fabrication of the enzyme-based biosensors and the analysis of phenolic compounds.
Laccase (Lac) has been widely used to construct electrochemical biosensors for phenolic and their derivatives, because it can catalyze the oxidation of phenolic compounds accompanied by the reduction of oxygen [1, 2]. The high stability and enzymatic activity of the bioelectrochemical interfaces play a crucial role in the construction of Lac-based biosensors. The immobilization of enzymes on solid supports is one of the effective strategies, which allows the recovering and reusing of enzyme for several reaction cycles [3, 4].
There is of intense interest in the construction of Lac-based biosensors using nanomaterials, due to their unique and particular properties . Silica materials, which can accommodate different dimensions of enzyme without affecting their biological activity, could be considered as suitable hosts for enzyme immobilization [6, 7]. For example, functionalized SBA-15 mesoporous silica was applied to immobilize Lac for the oxidation of a mixture of four phenolic compounds . In another work, Lac was encapsulated into thin silicate film deposited on the Au electrode . Moreover, magnetic mesoporous silica spheres were prepared to immobilize Lac as a promising support .
Multi-walled carbon nanotubes (MWCNTs), with high surface area and excellent biocompatibility, are also a promising candidate as the matrix material to incorporate enzyme and construct enzyme-based biosensors [11, 12]. Importantly, because of the low overvoltage and rapid electrode kinetics, MWCNTs have the ability to facilitate electron transfer of enzyme with the electrode . Therefore, MWCNTs have been employed as the supporting materials of Lac, such as the matrix based on MWCNTs-chitosan composite film , polyazetidine prepolymer-MWCNTs integrated system , and copper nanoparticles/chitosan/carboxylated MWCNT/polyaniline composite . Recently, researchers are committed to develop MWCNTs/silica nanocomposite as the immobilization materials of Lac, because they possess excellent properties of low toxicity and good electrocatalytic activity, and could provide a stabilizing microenvironment for Lac [16–18].
Screen-printed electrode (SPE) is a kind of planar sensor device with various substrates that are coated with layers of electroconductive and insulating inks at controlled thickness . Several works related to the biosensors construction have been reported based on the immobilization of Lac on the SPEs, which could be incorporated in portable systems as an alternative detection method for the direct in-situ analysis [20–22]. It is notable that the most common way to construct electrochemical interfaces is to drop conductive substrates onto the electrode surfaces [23, 24]. However, it is difficult to produce thin (<1 mm) layers and control the consistency of detection . While printing technology provides a convenient route to produce electrochemical sensors with consistent chemical performances based on the modification of functional conductive materials, such as conducting polymers , ionic liquid  and enzyme [26, 27]-doped conductive materials.
Reagents and apparatus
Laccase from Trametes versicolor (EC 126.96.36.199, 21.8 U mg-1), dopamine (DA), ascorbic acid (AA) and tetraethyl orthosilicate (TEOS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of analytical reagent grade purity and all solutions were prepared using deionized water obtained with a Mili-Q system (Millipore Co., Bedford, MA, USA). MWCNTs were supplied by Shenzhen Nanotech Port Co. Ltd. (Shenzhen, China) with a typical diameter of 10–30 nm and length of 5–15 μm, and their purity was 95-98%. Besides, silver paste, carbon paste, silver/silver chloride paste (Camnano Technology Ltd., Xuzhou, China), and insulating paste (Jujo Chemical Co., Ltd., Japan) were used to fabricate SPEs.
Scanning electron microscopy (SEM) results were obtained by using a Zeiss utra 55 field-emission SEM instrument (Zeiss, Germany). All the electrochemical measurements were performed with a CHI-1211A portable electrochemical workstation (Chenhua Instruments Co. Ltd., Shanghai, China). The measurements were performed at room temperature (~15°C).
Fabrication of MWCNTs/SPE
As the base electrodes for the printing process, SPEs with a standard three-electrode system and a 3.1 mm2 working area for each were fabricated according to the process described by our previous work [28, 29] with an AT-25P screen-printing machine (ATMA CHAMPENT. Corp., China). Compared to our previous publications, the working electrodes were printed using different mass proportions of MWCNT/carbon paste, drying at 100°C. The prepared MWCNTs/SPEs were then stored at 4°C until required.
Construction of the disposable biosensor
Silica spheres were synthesized according to the Stöber’s method . Typically, a solution of 5 mL of 33% ammonia solution was mixed with 50 mL of dry ethanol. After 3.14 mL of TEOS and 1.8 g of Milli-Q water was added in sequence, the solution was stirred to hydrolyze TEOS. After 12 h of stirring, a colloidal solution of silica spheres about 100 nm in diameter were obtained.
Before modification, the bare SPEs were pretreated in pH 7.0 potassium phosphate buffer solution (PBS) by applying an anodic potential of 2.00 V for 300 s. The synthesized silica spheres colloidal suspension was mixed with Lac (10.0 mg mL-1, prepared in 0.10 M pH 5.0 PBS) stock solution thoroughly in a volume ratio 2:3 for 24 hours. Then, 2.5 μL mixed solution of silica-Lac was coated onto the surface of the MWCNTs/SPE to form Lac/Si/MWCNTs/SPE as the disposable biosensor. After the solvent evaporated, the constructed biosensor was washed with deionized water to remove excess Lac. For comparison, Lac modified SPE (Lac/SPE), Lac modified MWCNTs/SPE (Lac/MWCNTs/SPE), and silica spheres modified MWCNTs/SPE (Si/MWCNTs/SPE) were fabricated with the similar steps. All of the modified electrodes were stored at 4°C.
Results and discussion
Morphology characterization of the disposable biosensor
Electrochemical properties of the disposable biosensor
The quinones formed in reaction (1) are usually electrochemically active and subsequently re-reduced on the surface of the electrode at the appropriate potentials. DA, as a typical model of o-benzenediol, follows this reaction mechanism. Then, Lac is used to initialize the sequential intramolecular cyclization reactions of DA, including a deprotonation reaction (Figure 1, reaction 2), an intramolecular cyclization process (reaction 3), and a disproportionation reaction and/or oxidation (reaction 4). The finally formed 5,6-dihydroxyindoline quinone is readily electrochemically reduced at SPE . On the basis of these reaction properties of DA, the non-oxidative electrochemical approach can be proposed for the determination of DA by measuring the cathodic current of 5,6-dihydroxyindoline quinone at a negative potential (−0.158 V).
The results of Figure 3B and C show that symmetrical redox couple of DA at the Lac/SPE and the Lac/MWCNTs/SPE with the potential difference between anodic and cathodic peaks (ΔEp) are 0.051 V and 0.044 V, characteristic of a two-electron and two-proton quasi-reversible redox process of DA at both SPEs . No cathodic peak at around −0.150 V is found at these SPEs. The results demonstrate that the Lac/SPE or the Lac/MWCNTs/SPE does not have any appreciable electrocatalytic activity to DA based on a non-oxidative electrochemical approach, implying the direct immobilization of Lac on bare SPE or MWCNTs/SPE is not successful. Moreover, the electrocatalytic features of the Si/MWCNTs/SPE to DA are similar to those of the Lac/SPE and the Lac/MWCNTs/SPE in terms of the anodic (0.057 V) and cathodic (0.015 V) peak potentials (blue curve in Figure 3D), and there is still no cathodic peak at around −0.150 V appearing at this Si/MWCNTs/SPE. However, after immobilizing Lac on the surface of the MWCNTs/SPE with silica spheres, the cathodic peak caused by the enzymatic oxidation of DA appears at around −0.158 V (red curve in Figure 3D). Obviously, this process is ascribed to the two-electron and two-proton quasi-reversible redox process of 5,6-dihydroxyindoline quinone, and implies that the Lac has been immobilized on the Lac/Si/MWCNTs/SPE stably with a good biocatalytic activity . Furthermore, the introduction of Lac makes the measurement of DA through cathodic current at negative potential (around −0.150 V) achieve, which avoiding the interference of other electroactive species whose oxidized potentials are very close to DA, by measuring the oxidation current of DA at a positive potential. These phenomena substantially demonstrate that the disposable biosensor can show an excellent electrocatalytic activity to DA based on this non-oxidative electrochemical approach. On one hand, the biosensor could retain the bioactivity of Lac to a large extent by immobilization of Lac with silica spheres on the MWCNTs/SPE. On the other hand, silica spheres and MWCNTs can both provide large loading area for Lac by their high specific surface area. The above results also imply that the Lac immobilized on the surface of Si/MWCNTs/SPE might provoke the drastic conformation change of Lac which is in favor of the active sites of enzyme approaching the SPE. However, if cross-linking reagents were used to immobilize Lac, this maybe promotes a high degree of reticulation with Lac that blocks the process .
The effect of pH on the electrochemical properties of the disposable biosensor
The effect of the amount of MWCNTs on the electrochemical properties of the disposable biosensor
Determination of DA using the disposable biosensor
The response properties of the disposable biosensor to DA
Linear range (μM)
Detection limit (μM)
Sensitivity (μA mM-1 cm-2)
1.3 to 85.5
2.787 × 103
Stability, reproducibility and repeatability of the disposable biosensor
One of the most critical issues in constructing biosensor is the avoidance of enzyme immobilized on the surface of the electrode leaking into the solution. In this study, the stability of Lac/Si/MWCNTs/SPE was investigated by recording cathodic peak current responses of the Lac/Si/MWCNTs/SPE at different sweep segments in 0.10 M PBS (pH 5.0) containing of 60.0 μM DA. It was found that the current responses stayed at the same level after 20 sweep segments, indicating that Lac was immobilized stably on the SPE. The results further proved that the proposed immobilization method was effective and it was not necessary to use any other cross-linking reagents. The possible reason may be due to the large loading area, good biocompatibility and the stabilizing property provided by silica spheres and MWCNTs, attributing to their porous and three-dimensional architecture. The nanosized pores on silica spheres and MWCNTs could act as small cages surrounding the Lac, consequently offering a protective chemical microenvironment which is similar to the microenvironment near enzyme in biological cells. Furthermore, the interconnected pores and a well-defined three-dimensional network of the proposed immobilization matrix can prevent Lac from leaching into the solution while allow free diffusion between the matrix and product molecules from/to the catalytic active sites. Therefore, even if in the absence of cross-linking reagents, the developed biosensor can still show good stability during the detection [35, 36].
To verify the reproducibility of the disposable biosensor, five different Lac/Si/MWCNTs/SPEs fabricated by same steps independently were chosen randomly from 50 pieces of store SPEs. The R.S.D. for the cathodic peak current responses to 60.0 μM DA was 6.5%, meaning that the constructing procedures were reliable and the modified SPEs had a good reproducibility. In addition, the same Lac/Si/MWCNTs/SPE was used to detect DA for five times successively. As a result, the R.S.D. value was 4.7%, showing a good repeatability.
The storage stability of the disposable biosensor was also investigated. After 10 and 30 days saving at 4°C, the current response of Lac/Si/MWCNTs/SPE reached to 91.0% and 86.0% of the initial response respectively in the 60.0 μM DA solution. The good stability may ascribe to the effective protection of the bioactivity of Lac due to the consistent stability of silica spheres and the biocompatible microenvironment provided by silica spheres and MWCNTs.
A novel disposable biosensor has been successfully constructed on MWCNTs/SPE by immobilizing Lac with silica spheres. Due to the large specific surface area and excellent biocompatibility of MWCNTs and silica spheres, the biosensor can effectively provide a suitable microenvironment for the immobilization of Lac and exhibit a good electrocatalytic performance for DA. In addition, based on a non-oxidative electrochemical mechanism, the biosensor enables the in situ determination of DA with good sensitivity, selectivity and reproducibility. In summary, the proposed approach of enzyme immobilization shows a great potential for the construction of biosensors without using cross-linking reagents, and the constructed biosensor displays an excellent analytical performance for phenolic compounds in a rapid and cost-effective way.
This research was supported by the National Science Fund for Distinguished Young Scholars (No. 21125522) and Natural Science Foundation of China (Grant No. 21007015).
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