Volume 3 Supplement 1

4th German Conference on Chemoinformatics: 22. CIC-Workshop

Open Access

Molecular modeling studies of lipase-catalyzed β-lactam polymerization

  • I Baum1,
  • LA Haller1,
  • LW Schwab2,
  • K Loos2 and
  • G Fels1
Chemistry Central Journal20093(Suppl 1):P57

https://doi.org/10.1186/1752-153X-3-S1-P57

Published: 05 June 2009

Enzymatic polymerization has emerged over the last 5 years as a field of considerable interest and commercial promise. The reaction proceeds with high regio-, enantio-, and chemoselectivity under relatively mild conditions. Enzymes have been used so far to synthesize polyesters, polysaccharides, polycarbonates, polyphenols, polyanilines, vinyl polymers, and poly-amino acids [1]. Particularly, lipase B of Candida antarctica immobilized on polyacrylic resin (Novozyme 435) has proven to be a very versatile catalyst and has successfully been used for the synthesis of polyesters from various substrates [2][3][4]. Little, however, has been reported on the enzyme catalyzed synthesis of polyamides [5].

While it has been shown that nylons can chemically be produced from the corresponding amino acids or by anionic ring-opening polymerization of 5–13 membered unsubstituted lactams, poly-β-alanine has not yet been obtained by either polymerization of β-alanine or β-lactam (2-azetidinone). Using lipase B of Candida antarctica we have recently been successful in the production of unbranched poly-βalanine starting from unsubstituted β-lactam [6].

Here we report preliminary molecular modeling studies of the lipase catalyzed ringopening polymerization of β-lactam towards an understanding of the underlying enzymatic mechanism. We can show that amide formation initially follows the well-known enzymatic acylation of Ser105 by β-lactam using Asp187 and His224 of the catalytic centre and Thr40 and Gly106 as oxy-anion hole. The elongation of the chain, however, utilizes different parts of the active site. The mechanism is only applicable for β-lactam and can not be utilized by β-alanine and suggests a reasoning for the experimental finding that β-alanine can not be polymerized enzymatically but rather inhibits the polymerization in a copolymerization experiment with β-lactam and β-alanine.

Authors’ Affiliations

(1)
Department of Chemistry, Faculty of Science, University of Paderborn
(2)
Department of Polymer Chemistry and Zernike Institute for Advanced Materials, University of Groningen

References

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Copyright

© Baum et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.