Determining the chemical activity of hydrophobic organic compounds in soil using polymer coated vials
© Reichenberg et al 2008
Received: 07 February 2008
Accepted: 06 May 2008
Published: 06 May 2008
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© Reichenberg et al 2008
Received: 07 February 2008
Accepted: 06 May 2008
Published: 06 May 2008
In soils contaminated by hydrophobic organic compounds, the concentrations are less indicative of potential exposure and distribution than are the associated chemical activities, fugacities and freely dissolved concentrations. The latter can be measured by diffusive sampling into thin layers of polymer, as in, for example, solid phase micro-extraction. Such measurements require equilibrium partitioning of analytes into the polymer while ensuring that the sample is not depleted. We introduce the validation of these requirements based on parallel sampling into polymer layers of different thicknesses.
Equilibrium sampling devices were made by coating glass vials internally with 3–12 μm thick layers of polydimethylsiloxane (PDMS). These were filled with slurries of a polluted soil and gently agitated for 5 days. The concentrations of 7 polycyclic aromatic hydrocarbons (PAHs) in the PDMS were measured. Validation confirmed fulfilment of the equilibrium sampling requirements and high measurement precision. Finally, chemical activities of the PAHs in the soil were determined from their concentrations and activity coefficients in the PDMS.
PAHs' thermodynamic activities in a soil test material were determined via a method of uptake into PDMS. This can be used to assess chemical exposure and predict diffusion and partitioning processes.
Chemical activity and the closely related fugacity were suggested as measures of chemical behaviour and affinity by G. N. Lewis in his now century old formulation of chemical thermodynamics . Since then they have been used to understand, predict and describe physico-chemical phenomena such as osmosis , mineral stability  and cholesterol biosynthesis [4, 5]. That the chemical activity of a hydrophobic organic soil pollutant is important for its bioavailability has recently been proposed . One reason for this is that partitioning occurs spontaneously down gradients in chemical activity. Equilibrium partitioning is defined by equal chemical activities, forming the basis for equilibrium partitioning theory  and related models (e.g. [8, 9]). All of which is taken to contend that the mentioned thermodynamic quantities can help in the prediction of chemical bioaccumulation and toxicity . Despite this promising perspective we found few published methods to determine the chemical activity [11, 12] or fugacity [13–15] of hydrophobic organic pollutants in environmental samples.
where γ denotes the activity coefficient and C the concentration of the analyte in the polymer (subscript P). At equilibrium, the analyte concentration in the polymer reveals new and important information about the sample.
Clearly, when compared to the more traditional analytical extraction techniques, equilibrium sampling differs in both means and ends. Not surprisingly they also present other challenges, and method performance must be evaluated differently. For example, Soxhlet extractions used in quantitative analytical methods are designed for complete analyte recovery, and tend to be sample destructive yielding "dirty" extracts that require further work-up before analysis. For such exhaustive extractions, it is well established that analyte recovery during extraction and clean-up can be validated by internal standards. Equilibrium sampling, in contrast, dictates that the sample must remain intact during the procedure. For example, grinding , heating and soaking soil samples in organic solvents are to be avoided as far as they destroy the matrix and might alter the chemical activity to be measured. Likewise, it must be assured that the ESD polymer is not damaged by abrasion, or its surface fouled by soil particles during the agitated equilibration procedure. These special criteria for valid equilibrium sampling are known, but inadequately addressed in practice.
Equilibrium sampling has some unusual criteria, and reliability could be gained from an integrated validation of (1st) equilibrium, (2nd) negligible depletion of sample and (3rd) negligible impact of other experimental artefacts such as adsorption on the polymer surface. This is especially important when long equilibration times (days) make separate validation experiments costly or the polymer phase is given a large surface area to volume (A/V) ratio to speed up equilibration. However, we could not find any published equilibrium sampling methods able to fulfil these validation requirements.
Diffusive samplers with multiple A/V ratios can be used to confirm equilibrium without time series measurements [17, 21]. Mechanistic arguments show that the A/V-approach allows not only validation of equilibrium, but extends to disclosing other potential artefacts such as sample depletion and polymer surface adsorption. The subsequent discussion explains the benefits of equilibrium sampling with devices of multiple coating thicknesses.
Many different ESD formats have been applied, including PDMS coated glass fibres , low-density polyethylene strips [23, 24], polyoxymethylene strips , poly(ethylene-vinyl acetate) films  and liquid-filled, hollow-fibre membranes , each with their specific operational window. Other alternatives include the Twister® (sorptive stir-bar) and the Immobilised Liquid Extraction™ Caps. Very recently Minhas et al. published work on vials internally coated with poly(ethylene-vinyl acetate) . This integration of glass container with the sampling phase allows soil and sediment samples to be transported and stored, for example, while equilibrating with the polymer coating. All the while minimising sample handling, increasing work efficiency and reducing worker exposure.
The limitations of existing ESDs and theoretical arguments suggest that equilibrium sampling with multiple coating thicknesses integrated within a sample container can provide validated and simplified measurements of chemical activity, fugacity and freely dissolved concentration. The aim of this paper is therefore to present ESDs consisting of μm-thick PDMS coatings of multiple thicknesses and their application to the measurement of chemical activity of PAHs in soil.
The ESDs consist of PDMS layers deposited on the internal wall of sample glass vials. The ESDs are equivalent in all respects apart from the thickness of the PDMS. This in vial-sample preparation minimises sample handling, and parallel application of the different thicknesses allows validation of the equilibrium sampling to measure chemical activity. The procedure consists of three parts:
1. Vials with 3–12 μm thin layers of PDMS are equilibrated with samples of the investigated soil.
2. Solvent extracts of the PDMS are analysed for the content of PAHs to determine C PDMS.
3. The chemical activity is found as the product of C PDMS and an activity coefficient (Equation 1).
Measured equilibrium partitioning concentration in PDMS (C PDMS), chemical activity (a) and concentration (C soil) of seven PAHs in the test soil
CPDMS (± SD) a
a n = 9-8
b dry matter
Method Limits of Quantification (LoQ)
Depletion of sample
Values of C PDMS can be applied in many different ways without any further conversion. Gradients in C PDMS follow the potential for diffusion and measured values should fall in the direction of passive transport gradients within or between environmental compartments. For example, as predictor of PCB bioaccumulation into worms, C PDMS is directly comparable between both dissimilar sediments  and soils . Likewise, C PDMS can be used to monitor environmental processes in time, such as the effect of aging or the (bio)degradation of PAHs in soil. A decrease in C PDMS is sufficient to show a decrease in chemical activity.
This calibration towards C free has already been covered in several publications (e.g. [17, 22, 31, 32]), and the present paper focuses on the determination of chemical activities from C PDMS (Equation 1).
Melting points (Tm), crystal activities (a xstal), solubilities (S(s)MeOH) and activity coefficients (γMeOH) in methanol. Methanol-PDMSe partition ratios (K PDMS/MeOH) and activity coefficients (γPDMS) in the PDMS for 12 PAHs
a xstal a, b
S(s)MeOH (± SD) b, c
K PDMS/MeOH e
γPDMS e, f
a Equation 4
b T = 298 K (25°C)
c n = 3
d γMeOH = axstalS(s)MeOH-1
e Silastic® PDMS
f Equation 3
The practice of ESD calibration via methanol partitioning standards may prove valuable in the future. Already now, methods to conduct multi-compartment studies including equilibrium sampling measurements are available [14, 22, 23, 36]. However, these methods use different polymers and this may confound a comparison of results. For example, the inter-calibration of two commercial PDMS formulations, Silastic® and SSP-M823, revealed an almost 40% difference in their partitioning properties (Table A in Additional File 1). This kind of information is important for comparing measurements from different ESDs.
The in vial-ESD format and A/V-approach has unique and important features. Most important is the integrated QA/QC of the measurements. This extends the applicability towards very difficult or very dirty samples that might cause problems during equilibration or analysis due to their composition. Examples of troublesome sample components include soot particles with a high PAH content  and (micro)droplets of non-aqueous phase liquids (NAPLs) that may foul the PDMS surface. But, when using the in vial-ESD format, equilibrium sampling flaws can be inspected by plotting na against VPDMS. This integrated validation makes the method reliable at its applicability limit, whereas other equilibrium sampling methods require a greater margin of safety. Second, the integration of thin polymer layers within sample vials  reduces the risk of measurement error. Handling procedures are minimised as sample material can be secured, transported, stored and equilibrated, under seal and in a single container. The subsequent liquid extract of the PDMS makes the method compatible with many standard analytical procedures. For example, addition of internal standards, clean-up and/or pre-concentration steps can be included in future equilibrium sampling method developments. The in vial-ESDs are both practical and versatile. Third, the design of the devices is also adaptable. The ESD dimensions can be tuned towards faster equilibration by reducing the polymer coating thickness . Alternatively, detection limits can be lowered by increasing the VPDMS to sample a greater amount of analyte for detection. All of these advantages make ESDs with multiple A/V ratios attractive.
3–12 μm layers of PDMS were prepared in 20 mL glass vials by dispersion coating in the following manner. The appropriate mass of medical adhesive, Silastic® PDMS Silicone Type A (Dow Corning, Seneffe, BE; density 1.15 kg/L ) paste was dispersed in 10 times its volume in n-pentane by wrist shaking and sonication. The dispersion was filtered (glass fiber, 0.7 μm nominal, Pall Corp., MI) and diluted in a geometric series. All polymer dispersions were used at the day of preparation and should be prepared using disposable glassware and materials to avoid inadvertent formation of insoluble polymer layers.
After careful tare on an analytical balance (Sartorius BP 210S, d = 0.1 mg), the vials to be coated were placed horizontally on a roller-mixer (Stuart sci., Stone, UK). While rotated (33 rpm) 3.0 mL of the appropriate dispersion was added to each vial. The solvent was evaporated under a gentle stream of nitrogen and the vials were left to polymerise for 72 h in a fume hood. Siloxane oligomers were removed from the cross-linked PDMS by three extractions with ethyl acetate (3 mL, 10 min vigorous shaking). The vials were again balanced to determine the mass of PDMS now covering their inner sidewall surface.
PDMS-coated vials with different coating thicknesses were filled with 18–20 g of the test soil (n = 9). 10 mL aqueous solution of sodium azide (0.5 g/L) was added to each vial to inhibit microbial activity and to create a soil suspension for the sampling. Vials were capped with metal lined screw caps and placed horizontally in a customised holder rotated (6 rpm) on a roller-mixer for 122 h.
Soil content was removed, the vials were rinsed with a little distilled water on a whirly mixer before being wiped internally with lint-free tissue. To each vial was then added 1.00 mL methanol. The vials were again capped and rotated for 12 h. The methanol extracts were collected and stored in freezer (-20°C) until analysed for PAHs.
Methanol extracts of PDMS were analysed for PAHs by HPLC-fluorescence detection (Agilent 1100 system with G1321A FLD (Ex. 260 nm; Em. 350, 420, 440 and 500 nm). Separation column: CP-Ecospher 4 PAH (Varian Inc., Palo Alto, CA) operated at 0.5 ml/min (28°C, 10 μL injection); Mobile phase: methanol, SUPER-Q treated water (Millipore, MA); Gradient (in %methanol, by weight): t = 0 min 80%; t = 5–30 min linear gradient 80–100%; t = 30–45 min 100%. Quantification was accomplished by a five-point external standard curve. Extract analysis was carried out within 2 weeks after sampling.
Solubilities were measured by placing excess PAH crystals together with methanol in PTFE-sealed glass vials and equilibrating these in a thermostated water bath at 25.0 ± 0.1°C for at least 3 days. Attainment of the solubility limit was verified by repetitive measurements on consecutive days. Aliquots of the saturated methanol solutions were passed through 0.2 μm PTFE-filters into volumetric flasks and quantitatively diluted with methanol to reach suitable concentrations before analysis by the HPLC-FLD method described above.
Partition ratios (K PDMS/MeOH) between Silastic® PDMS polymer and methanol was measured in a separate experiment. First, PDMS sheets were prepared by filling a polyethylene mold (0.25 mm high) with Silastic® paste. The top surface was pressed even, and covered with wet tissue and a glass plate. After one week, the cross-linked PDMS was cut into sheets weighing approximately 1 g, Soxhlet extracted (ethyl acetate, 100 h) and then washed with methanol. A clean sheet and an excess of methanol solution containing all the analyte PAHs (approx. 25 μg/L) were placed in a sealed amber glass container. After equilibration by shaking for > 1 week at 20°C, the methanol and PDMS were separately taken for extraction and analysis of the PAH contents by GC-MS as described in . The respective, volume based, concentrations in the Silastic® PDMS sheet and the methanol solution were divided to calculate the partition ratios.
This study concludes that vials containing thin layers of PDMS can be used as equilibrium sampling devices. The methodology of parallel sampling in devices with different thickness PDMS layers has unique and important features. Most important is an integrated QA/QC of the procedure. When conducted on a soil test material, results were confirmed as precise and valid measurements of equilibrium partitioning concentrations, C PDMS. These were then, by way of a separate calibration experiment, expressed as chemical activities of individual PAHs in the soil.
A risk assessment of the pollution in the test material soil is warranted by the present soil quality criterion for PAHs in Denmark. The legislation dictates that the sum of fluoranthene, benzo [a]pyrene, benzo [k]fluoranthene, and indeno [1,2,3-cd]pyrene may not exceed 4 mg/kg dry soil .
Several concerns regarding the risk posed by the PAHs in the polluted soil can be assessed with the chemical activities reported in Table 1. First, a soil quantifies the chemical pollutant's potential for partitioning and distribution to other materials in contact with the soil. This makes the activity relevant because it is first after release and transfer to a susceptible target that a PAH may cause detriment of health or effects on the environment. Second, a soil provides a measure for comparison of chemical contamination levels. The activity reflects a chemical's affinity (e.g. partial molar free energy of sorption) to the soil material. Soils are dynamic, heterogeneous and unpredictable geosorbents. Therefore, C soil is harder to interpret and compare than are concentrations in a well-defined, stable and homogenous phase such as PDMS. In consequence, equilibrium sampling measurements are useful because the results can easily be compared to the levels in other measured soils, in air, water or biological tissue, as well as to effect levels determined in toxicity studies, whenever such are available.
PAHs of ≥98% purity were obtained from Sigma-Aldrich (Copenhagen, DK) or Cambridge Isotope Lab. (Andover, MA).
P.a. grade n-pentane, ethyl acetate, sodium azide and HPLC-grade methanol were obtained from Merck (Darmstadt, DE).
The PAH contaminated soil were excavated from a former manufactured gas plant site (Gaswerk Tiefstark, Klosterwall 2, Freie Hansestadt Hamburg, DE) and then compost treated by A/S Bioteknisk Jordrens (Esbjerg, DK) prior to this study. It contained 14 % water, 5–7 % organic carbon, 11–16 % silt, 3–4 % clay and weathered coal-tar, petroleum residues, soot and ash.
The bioremediation of the soil had removed a fraction of the PAHs; whereas those associated with the black carbon  likely remained. The residual total concentration of the 16 EPA PAHs was 120 mg/kgsoil. This test material was selected on account of the PAH-containing soot that is claimed to complicate equilibrium sampling . Prior to sampling, the soil was sieved to 2 mm (steel mesh) and manually homogenized.
The qualification criteria for PAHs extracted from the ESDs included retention times within 5% of a known sample and correct fluorescence emission wavelength(s). Signal integration was performed with HP Chemstation software (A.06.03, Agilent Tech.) and corrected by hand as necessary. About 7% standards and solvent blanks were included in the analytical sample sequences. ESD blanks were considered unnecessary since positive blank values would be eliminated during the equilibration. The measured results were tested by least squares linear regression of nPAH on VPDMS, forced through the origin. An r2 > 0.90 goodness of fit, was set as a necessary but not sufficient equilibrium sampling criterion. One outlier in the data set for anthracene was defined as the measured value of nANT differed by a factor > 2 from the predicted (n = 9). Equilibrium concentrations in PDMS (C PDMS, Table 1) were calculated as the arithmetic mean of the measurements (n = 9-8).
where a xstal is the activity of the PAH in its crystal state, S(s)MeOH the solubility in methanol and K PDMS/MeOH is the Silastic® PDMS-methanol partition ratio.
List of abbreviations
Polycyclic Aromatic Hydrocarbon
Solid Phase Micro-Extraction
Eqilibrium Sampling Device
(surface)Area to Volume ratio
Limit of Quantification
Quality Assurance/Quality Control
Chemical (thermodynamic) activity
Activity coefficient in phase i
Partition ratio between phases i and j
Concentration in (phase) i
Melting point temperature
Volume of phase i
Amount of analyte
Solubility (solid) in phase i
number of replicate determinations
Goodness of fit
Ellen Christensen (NERI, DK) measured the soil concentrations and Margit Fernquist (NERI, DK)measured solubilities. This research was funded by the Danish Technical Research Council (Young Research Leader grant), the Danish Research School of Environmental Chemistry and Ecotoxicology and the European Commission within the FP 6 Integrated Project "Novel Methods for Integrated Risk Assessment of Cumulative Stressors in the Environment" (NOMIRACLE, GOCE-003956).
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